应用离心管技术以异体微粒软骨脱细胞基质为支架体外构建组织工程软骨  

Experimental study of tissue engineered cartilage construction with scaffold of allogenic cartilage microparticle acellular tissue matrix

作　　者：韩雪峰[1] 杨大平[1] 张颖[2] 郭铁芳[1] 方冬云[1] 

机构地区：[1]哈尔滨医科大学附属第二临床医学院整形外科,150086 [2]哈尔滨医科大学附属第二临床医学院病理科,150086

出　　处：《中华风湿病学杂志》2005年第1期1-3,i001,共4页Chinese Journal of Rheumatology

基　　金：国家自然科学基金资助项目(3017090);黑龙江省杰出青年基金资助项目(JC-02-07);黑龙江省教育厅基金资助项目(10511039)

摘　　要：目的将软骨细胞与异体软骨微粒脱细胞基质相结合,构建组织工程软骨.方法分别利用氯化钾、胰蛋白酶和乙二胺四乙酸钠对绵羊关节软骨进行脱细胞,并制成直径0.100～0.154 mm的微粒.先分离异体关节软骨细胞并进行体外扩增,再将异体软骨细胞与软骨微粒脱细胞基质混合培养,离心后应用离心管体外培养.结果本脱细胞方法可以使关节软骨细胞完全脱落,且软骨细胞紧紧围绕于异体软骨微粒脱细胞基质四周,生长和分泌功能良好.结论软骨细胞与异体软骨微粒脱细胞基质有良好的生物相容性,可于体外形成软骨样组织.Objective To construct tissue engineered cartilage by combining allogenic cartilage microparticle acellular tissue matrix and chondrocytes. Methods Cartilage microparticle acellular tissue matrix from sheep articular cartilage were prepared with KCl, trypsin and EDTA, the diameter of which was from 0.100 mm to 0.154 mm. Allogenic chondrocytes were isolated and cultured in vitro. Finally, chondrocytes and cartilage microparticle acellular tissue matrix were mixed and cultured in centrifuge tube in vitro. Results The acellularization procedure resulted in a complete removal of the original cells. Allogenic microparticles were circled by chondrocytes whose growth and secretion were good. Conclusions Allogenic cartilage microparticles acellular tissue matrix possesses satisfactory biocompatibility to chondrocytes. Cartilage tissue can be constructed by this procedure in vitro.

关 键 词：离心管技术 异体微粒 脱细胞基质 支架 构建 组织工程 

分 类 号：R318.1[医药卫生—生物医学工程]