帕金森病大鼠同种异体脑内移植免疫反应的实验研究  

Exprimental research on immunological rejection of brain tissue allograft

作　　者：韩洋[1] 曾水林[1] 王磊[1] 雷志年[1] 

机构地区：[1]东南大学医学院神经生物学研究所,江苏南京210009

出　　处：《细胞与分子免疫学杂志》2005年第1期83-85,89,共4页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的: 观察帕金森病(Parkison' s disease, PD)模型大鼠同种异体脑内移植是否存在免疫反应以及免疫反应的特点.方法: 取新生SD大鼠腹侧中脑(ventral mesencephalon, VM)组织制成单细胞悬液, 借助脑立体定位仪移植到SD大鼠PD模型的纹状体内, 于2、 4、 6 wk时采用行为学检测后分批处死, 再行HE、酪氨酸羟化酶(TH)和MHCⅡ抗原免疫组化染色.结果: PD模型移植组2 wk时, MHCⅡ表达明显增高(P＜0.05), 4 wk时开始下降, 6 wk以后消失; 2 wk时移植区可见少量TH阳性神经元, 4～6 wk数量显著增加(P＜0.05).假手术组在2 wk时见少量MHCⅡ阳性细胞(P＞0.05), 4 wk时消失, 各时间点都未见TH阳性神经元; 对照组在各时间点都未见TH阳性神经元和MHCⅡ阳性细胞.各实验组各时间点行为学检测无明显改变(P＞0.05).结论: 同种异体脑内移植是存在免疫排斥反应的, 这种反应可能是由MHCⅡ抗原不同而诱发的, 免疫反应与移植物内TH神经元的存活和发育密切相关, 同种异体脑内移植加用免疫抑制剂还是必要的.AIM: To investigate the occurrence of rejection in brain tissue transplantation. METHODS: Ventral mesencephalon(VM) single cell suspensions from newborn rats were allografted into the striata of Parkinson’s disease model recipient rats. Surviving animals were sacrificed at 2, 4 and 6 wk after transplantation, and the brain tissues were stained with HE or immunocytochemically to inspect the expression of tyrosine hydroxylase (TH) and major histocompatibility complex classⅡantigens (MHCⅡ). RESULTS: High MHCⅡ expression was observed within and around the allografts compared with control at 2 wk after grafting. MHCⅡ expression then decreased at 4 wk and at 6 wk only few immunopositive cells remained. The number of TH positive neurons was low at 2W, but increased at 4 and 6 wk after transplantation. CONCLUSION: The brain is not an “immunologically privileged site” and graft rejection exists in brain tissue transplantation. This rejection maybe induced by MHCⅡ. Therefore it is necessary to use immunosuppressants in brain transplantation.

关 键 词：同种异体 脑移植 主要组织相容性Ⅱ类抗原 酪氨酸羟化酶 免疫排斥 

分 类 号：R392.11[医药卫生—免疫学]