汞、铬和锰化合物雌激素样作用的实验研究  

Experiment study on the estrogen-like effect of compounds of Mercury、 Chromium and Manganese

作　　者：王亚东[1] 陈小玉[1] 吴逸明[1] 许东[1] 许玉宝[1] 

机构地区：[1]郑州大学医学院,郑州450052

出　　处：《卫生研究》2005年第1期49-51,共3页Journal of Hygiene Research

基　　金：河南省自然科学基金资助项目(No.0311041700)

摘　　要：目的为评价汞、铬和锰化合物雌激素样作用。方法选用氯化汞、硫酸锰、氯化铬、三氧化铬4种化合物进行MCF7人乳腺癌细胞增殖试验和雌激素受体竞争结合试验。结果10-9×10-5molL氯化汞能诱导MCF7人乳腺癌细胞增殖,10-7molL氯化汞使MCF7人乳腺癌细胞增殖达最大,其增殖作用可被雌激素受体拮抗剂ICI182780完全阻断;但不影响雌二醇与雌激素受体结合。硫酸锰、氯化铬、三氧化铬均不能诱导MCF7人乳腺癌细胞增殖,且不影响雌二醇与雌激素受体结合。结论氯化汞可能通过雌激素受体介导而表现出雌激素样作用;硫酸锰、氯化铬、三氧化铬不具有雌激素样作用。Objective In order to study estrogen-like effect of compounds of Mercury,Chromium and Manganese. Methods Mercury Chloride, Manganese Sulfate, Chromium Chloride and Chromium trioxide were selected to perform proliferation assay of MCF-7 human breast cancer cells and binding assay of estrogen receptor for rat uterine. Results Mercury Chloride could stimulate the proliferation of MCF-7 cell, the maximal increase of MCF-7 cells was measured by Mercury Chloride at 10^-7 mol/L. The effect was blocked completely by a pure antigestrogen ICI182,780;but Mercury Chloride could not bind to ER by competing with E2. Chromium Chloride, Chromium Trioxide and Manganese Sulfate were not able to stimulate the proliferation of MCF-7 cell and bind to ER through competing against ~ 3H-E2 in vitro. Conclusion Mercury Chloride exhibited the estrogenicity through binding and activating ER; Chromium Chloride, Chromium trioxide and Manganese Sulfate did not show any estrogenicity.

关 键 词：汞 锰 铬 MCF-7细胞增殖 雌激素受体 

分 类 号：R994.3[医药卫生—毒理学] R737.9[医药卫生—药学]