Tenascin-C在瘢痕疙瘩和增生性瘢痕中的基因表达研究  

The expression of Tenascin-C mRNA in keloids and hypertrophic scars

作　　者：韩春茂[1] 贺肖洁[1] 马奇[2] 

机构地区：[1]浙江大学医学院附属第二医院烧伤科,杭州310009 [2]浙江大学医学院附属第二医院整形科,杭州310009

出　　处：《中华整形外科杂志》2005年第1期40-43,共4页Chinese Journal of Plastic Surgery

摘　　要：目的　探讨Tenascin C基因在瘢痕疙瘩和增生性瘢痕中的表达。方法　取正常成人皮肤组织RNA ,构建正义、反义Tenascin C(Tn C)mRNA探针 ,运用原位杂交技术 ,观测 10例瘢痕疙瘩、10例增生性瘢痕和 5例正常成人皮肤组织中Tn CmRNA的表达。结果　Tn CmRNA在正常皮肤表皮中无表达 ,真皮中表达稀少 ,局限于乳头真皮层的成纤维细胞和皮肤附属器 ;10例瘢痕疙瘩表皮均有表达 ,真皮分布较广 ,如成纤维细胞、血管内皮和皮肤附属器 ;Tn CmRNA在 3例增生性瘢痕表皮表达 ,7例无表达 ,真皮中表达与瘢痕疙瘩相同但较弱 ,比正常皮肤增多 ,但差异无显著性。结论　Tenascin CmRNA在瘢痕疙瘩表皮和真皮中有高表达。Objective To investigate the expression of Tenascin |C mRNA in keloids and hypertrophic scars. Methods Total RNA was isolated from normal adult skin.A cDAN fragment(base 5941—6481bp)of the full |length human Tenascin |C cDNA was synthesized by polymerase chain reaction and subcloned in pGEM |T |easy.Dioxigen |labeled anti |sense and sense probes were synthesized by using a Sp6/T7 RNA synthesis kit in the present of Dig |UTP in vitro.The samples were taken from keloids in 10,hypertrophic scars in 10 and normal adult skin in 5.The hybridization was performed with 4% paraformaldehyde |fixed and wax |embedded sections to detect the Tenascin |C mRNA. Results The Tenascin |C mRNA was negative in the normal adult epidermis and weakly located in the fibroblasts of the papiliary dermis and the epidermal adnexa.In all of the 10 keloid specimens,the Tenascin |C mRNA was positive throughout the epidermis and widely distributed in the dermis included in the fibroblasts,endothelial cells and epidermal adnexa.In the specimens of the 3 hypertrophic scars,the Tenascin |C mRNA was also positive in the epidermis,but in the other 7 cases,it became negative.In the dermis of the hypertrophic scar,the Tenascin |C mRNA was weaker than that in the keloid,but stronger than that in the normal skin. Conclusions The expression of Tenascin |C mRNA is markedly enhanced in the keloids. ;

关 键 词：TENASCIN-C 瘢痕疙瘩 增生性瘢痕 基因表达 

分 类 号：R622[医药卫生—整形外科]