褐飞虱对噻嗪酮抗性的遗传分析  

Genetic analysis of resistance to buprofezin in the brown planthopper, Nilaparvata lugens (Stl) (Homoptera: Delphacidae)

作　　者：庄永林[1] 沈晋良[1] 戴德江[1] 周威君[1] 

机构地区：[1]南京农业大学植物保护学院农药科学系

出　　处：《昆虫学报》2004年第6期749-753,共5页Acta Entomologica Sinica

基　　金：国家"九五"攻关项目 (96_0 0 5_0 1_0 7)

摘　　要：害虫的抗性遗传特性是影响其抗性发展的一个重要因子 ,也是制订抗性治理对策的重要依据。我们采用稻茎浸渍法测定了褐飞虱Nilaparvatalugens(St l)抗性和敏感亲本、正反交 (F1 、F′1 )、自交 (F2 、F′2 )及回交 (BC)后代 3龄若虫对噻嗪酮的剂量反应数据 ,研究了褐飞虱对噻嗪酮的抗性遗传特性。结果表明 :正反交后代的显性度分别为 - 0 31 5 3(F1 )和 - 0 376 3(F′1 ) ,表明抗性遗传为常染色体的不完全隐性 ;将自交及回交后代的剂量反应数据进行单个主基因假设的卡方 ( χ2 )检验 ,其卡方值分别为 4 2 1 1 (F2 )、5 1 4 4 (F′2 )及 93 5 7(BC) ,均大于 χ20 0 5=1 5 5 1 (df=8) ,表明其抗性是多基因控制的。The inheritance of pest-insect's resistance to insecticide is not only an important factor affecting the development of resistance but also the basis for formulating resistance management strategy. The inheritance of resistance to buprofezin in the brown planthopper, Nilaparvata lugens (Stl),was studied through assaying dose-response data of the third nymphs of resistant (R) and susceptible (S) parents, reciprocal crosses (F-(1) and F~′-(1)), self-bred (F-(2) and F~′-(2)) and backcross (BC) progenies to buprofezin with the rice-stem dipping method. The results showed that the main resistant gene was autosomal and incompletely recessive, with degree of dominance (D) -0.3153 (F-(1)) and -0.3763 (F~′-(1)); the chi-square analysis results of the dose-response data of F-(2), F~′-(2) and BC progeny was 42.11, 51.44 and 93.57 respectively, all higher than χ~2-(0.05)=15.51(df=8), indicated that buprofezin resistance in the brown planthopper appears to be controlled by two or more genes. The resistance management tactics of N. lugens to buprofezin is also discussed here.

关 键 词：褐飞虱 稻茎浸渍法 噻嗪酮 抗性遗传 

分 类 号：Q965.9[生物学—昆虫学]