日本血吸虫SIEA26-28kDa的抗雌虫生殖免疫作用——HGPRT是否是主要的靶抗原(英文)  

Anti-fecundity immunity induced by sIEA 26-28kDa antigens of Schistosoma japonicum:is HGPRT antigen a major target for the immunity?

作　　者：李文凯[1] 汪世平[1] 吕志跃[1] 彭先楚[1] 徐绍锐[1] 何卓[1] 周松华[1] 肖小芹[1] 曾少华[1] 戴橄[1] 余俊龙[1] 车宏丽[1] 

机构地区：[1]中南大学湘雅基础医学院血吸虫病研究室,中国长沙410078

出　　处：《中国人兽共患病杂志》2005年第1期1-4,M004,共5页Chinese Journal of Zoonoses

基　　金：国家"十.五"重大科技专项课题 (No .2 0 0 2AA2Z3 3 43 ) ;863国家血防重大专项 (No .2 0 0 4AA2Z3 5 3 0 ) ;教育部"985"行动计划专项资金,湖南省"十.五"重点学科建设专项经费 (No .2 0 0 1-2 0 0 7)联合资助~~

摘　　要：目的　观察日本血吸虫SIEA2 6 2 8kDa抗原的抗雌虫生殖作用。方法用AcA柱层析纯化日本血吸虫早期胚卵中的SIEA和SIEA2 6 2 8kDa成分 ,并以此纯化产物免疫BALB/c小鼠。将小鼠分为三组 ,分别用SIEA、SIEA2 6 2 8kDa和免疫佐剂免疫动物 ,然后进行尾蚴攻击感染。 4 6天后剖杀动物 ,冲虫 ,然后作虫荷测定及虫卵分类计数。结果　SIEA2 6 2 8kDa免疫组雌虫子宫内减卵率为 5 4 .8% ,与对照组比较有极显著性差异 ;该实验组动物肝脏和小肠组织内的虫卵数比对照组分别降低了 4 8.0 7%和 77.4 1% ,且以成熟虫卵数的下降较为显著 ,分别降低了 83.6 %和 93.3% ;粪卵数的下降幅度最为显著 ,达87.2 6 %。结论　SIEA2 6 2 8kDa可作为一种抗卵胚发育及抗雌虫生殖的候选抗原分子。To study the anti fecundity effect of SIEA 26 28 kDa antigens from Schistosoma japonicum, the soluble immature egg antigen (SIEA) and the SIEA26 28kDa components from the early embryo stages eggs were purified through an AcA column and used to immunize BALB/c mice. The mice divided into three groups were immunized with SIEA, SIEA26 28kDa and immunoadjuvant respectively followed by a schistosome challege infection. The worm burden, the differential count of eggs in the host tissues and the schistosomal uterus were performed 46 days after the infection. The result showed that the decrease rates of intrauterine eggs of the female worms were 54.8% after immunization with SIEA26 28kDa, with a statistical difference being highly significant comparing with the control group. The numbers of schistosome eggs in the animal liver and intestine tissues decreased by 48.07% and 77.41% , respectively. To a more remarkable extent, the numbers of the mature eggs in the liver and intestine dropped by 83.6% and 93.3%, respectively, but most remarkably, the fecal egg decrease rate went up to 87.26%. No similar results were observed in the controls. In conclusion, SIEA26 28kDa can be regarded as acandidate antigen used for the induction of anti embryonation and anti fecundity immunity.

关 键 词：日本血吸虫 靶抗原 对照组 虫卵 宫内 候选抗原 免疫组 雌虫 生殖 免疫动物 

分 类 号：R383.24[医药卫生—医学寄生虫学] S899.3[医药卫生—基础医学]