三种肾综合征出血热诊断方法的比较  

Comparision of three diagnostic methods for the detection of specific anti-bodies in hemorrhagic fever with renal syndrome

作　　者：史俊岩[1] 王舰[1] 牟玲[2] 王晓梅[2] 李妍[2] 罗恩杰[1] 

机构地区：[1]中国医科大学病原生物学教研室,沈阳110001 [2]沈阳市传染病院

出　　处：《中国人兽共患病杂志》2005年第1期32-33,M002,共3页Chinese Journal of Zoonoses

基　　金：沈阳市科委资助项目(2001419-01)

摘　　要：目的　探索一种更为敏感而特异的肾综合征出血热特异性抗体的检测方法。方法　采用免疫 -PCR、ELISA和IFAT三种方法检测 130份HFRS患者血清中抗HFRS IgG抗体。结果　免疫 -PCR方法的灵敏度为 78 5 % ,特异性为10 0 % ;ELISA法和IFAT法的灵敏度分别为 4 7 7%和 4 9 2 %。结论　免疫 -PCR方法的敏感性高于ELISA法和IFAT法 ,是适用于肾综合征出血热特异性抗体检测的好方法。To search for the more sensitive and specific methods for the detection of specific antibodies in hemorrhagic fever with renal symdrome (HFRS), three diagnostic methods, i.e. immuno PCR, ELISA and IFAT, were used to compare the efficiencies of detecting the anti HFRS IgG antibodies in serum samples of 130 patients with HFRS. It was proved that the immuno PCR method was the best method of choice with the sensitivity and specificity of 78.5% and 100% respectively; the next two methods were ELISA and IFAT with the sensitivities of 47.7% and 49.2% respectively. It concludes that the immuno PCR is a good method to detect the specific antibodies in hemorrhagic fever with renal syndrome.

关 键 词：肾综合征出血热 免疫-PCR ELISA IFAT 

分 类 号：R373.3[医药卫生—病原生物学]