鼻腔接种伤寒杆菌Fe-SOD的抗体应答及对鼠伤寒杆菌攻击的免疫保护作用  

Intranasal immunization with Salmmonenal typhi Fe-SOD causes antibody responses and protects mice against challenge with Salmmonenal typhimurium

作　　者：徐军发[1] 唐湘涓[1] 凌天翼[2] 唐俊杰[2] 

机构地区：[1]广东医学院医学临床免疫学教研室,湛江524023 [2]贵阳医学免疫学教研室

出　　处：《中国人兽共患病杂志》2005年第1期66-69,共4页Chinese Journal of Zoonoses

基　　金：湛江市科技攻关项目 (ZK0 12 3 )

摘　　要：目的　探讨鼻腔接种伤寒杆菌Fe SOD后血液和粘膜系统的抗体应答及免疫保护作用。方法　用IL - 1作为佐剂 ,将伤寒杆菌Fe SOD经鼻腔接种小鼠 ,检测小鼠血液及肠液中的抗体应答 ,并用鼠伤寒杆菌攻击小鼠 ,观察小鼠的存活情况 ,以确定免疫保护作用。结果　当用IL - 1作为佐剂时 ,经鼻腔接种伤寒杆菌Fe SOD的小鼠血液和肠液中可产生高水平特异性IgG和IgA ;而腹腔注射仅在血液中产生高水平特异性IgG。另外发现经鼻腔接种伤寒杆菌Fe SOD的小鼠在 2LD50 鼠伤寒杆菌攻击后 ,3d和 7d的存活率均明显高于对照组 (P <0 0 5 ) ,且以 5LD50 鼠伤寒杆菌攻击时 ,小鼠 7d的存活率明显高于对照组 (P <0 0 1)。结论　结果说明伤寒杆菌Fe SOD经鼻腔接种后可引起小鼠粘膜系统和血液中的抗体应答 ;对鼠伤寒杆菌的攻击可产生一定的免疫保护作用 ,也进一步说明了Fe SOD是沙门菌的共同保护性抗原。To observe the antibody responses and the active cross immuno protection against challenge with Salmmonenal typhimurium of intranasal administration with S. typhi Fe SOD in mice. The mice were immunized with S. typha Fe SOD intranasally with IL 1 as adjuvant. The titer of specific antibody against Fe SOD in sera and in lavage of intestines of mice was tested with ELISA. Then the mice were challenged with different doses of S. typhimurium, and the survival rates of mice were recorded to decide the immune protection of SOD.There are high level of specific IgG and IgA against Fe SOD in sera and lavage of intestines in mice intranasally administrated with S. typhi Fe SOD, but there are high level of specific IgG in sera only in mice immunized with S. typhi Fe SOD peritoneally. The survival rates of mice in the group immunized intranasally with S.typhi Fe SOD with IL 1 as adjuvant challenged with 2LD50 S. typhimurium were higher than that of control groups during a period of 3 days and 7 days, respectively, and the survival rates of mice in the experiment group challenged with 5LD50 S. typhimurium were also higher than that of control groups during a period of 7 days. It is concluded that intranasal immunization with S. typhi Fe SOD could causes antibody responses in sera and intestinal mucosa and it offers an active cross immuno protection to mice challenged with S. typhimueium. The results indicate that SOD is the common protective antigen of Salmonella.

关 键 词：伤寒杆菌 鼠伤寒杆菌 FE-SOD 粘膜 抗体应答 免疫保护作用 

分 类 号：R378.2[医药卫生—病原生物学]