猕猴组织工程骨异体植入后白细胞介素2及其受体的表达  

EXPRESSION OF INTERLEUKIN 2 AND IL-2 RECEPTOR AFTER IMPLANTED TISSUE ENGINEERED BONES CONSTRUCTED WITH ALLOGENEIC MARROW STROMAL STEM CELLS AND BIO DERIVED MATERIALS IN RHESUS MONKEYS

作　　者：阳富春[1] 杨志明[1] 李秀群[1] 周悦婷[1] 林凡[1] 秦廷武[1] 罗静聪[1] 解慧琪[1] 

机构地区：[1]四川大学华西医院生物治疗国家重点实验室:干细胞与组织工程研究室,成都610041

出　　处：《中国修复重建外科杂志》2005年第2期105-108,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金资助项目 (39830 1 0 0 ) ;国家高技术研究发展计划 (863)资助项目 (2 0 0 2 AA2 0 50 1 1)~~

摘　　要：目的　检测猕猴组织工程骨异体植入桥接节段骨缺损后体内白细胞介素 2 (interleukin 2 ,IL - 2 )及白细胞介素 2受体 (interleukin 2 receptor,IL - 2 R)的表达 ,从免疫学方面探讨同种异体细胞作为种子细胞构建组织工程骨的可行性。　方法　用猕猴骨髓基质干细胞 (marrow stromal stem cells,MSCs)体外诱导分化为成骨细胞 ,与人源生物衍生骨材料复合培养 ,构建组织工程骨 ,植入 15只免疫功能健全的异体猕猴体内桥接桡骨节段骨缺损作为实验组 ;用单纯生物衍生骨材料桥接对侧同样大小骨缺损作为对照组 ;分别于术后 1、2、3、6和 12周抽取静脉血 ,并行双侧局部组织取材 ,组织块作低温匀浆 ,用双抗体夹心 IL ISA法检测 IL - 2及 IL - 2 R的表达。　结果　实验组和对照组术后各时间点IL - 2及 IL - 2 R水平无统计学差异 (P>0 .0 5 ) ,IL - 2及 IL - 2 R水平在术后第 2周开始增高 ,第 2～ 6周维持高水平 ,6周后随时间的延长而下降。　结论　猕猴 MSCs和生物衍生骨材料复合构建组织工程骨 ,植入异体猕猴体内引发微弱的免疫反应 ,同种异体 MSCs可选择作为构建骨组织工程的种子细胞。Objective To explore the feasibility of allogeneic marrow stromal stem cells(MSCs) as seed cells to construct tissue engineered bone by detecting the expressions of interleukin 2(IL 2) and IL 2 receptor in rhesus monkeys after implanting these tissue engineered bones. Methods Engineered bones were constructed with osteoblasts which derived from allogeneic MSCs and bio derived materials in vitro, and then were implanted to bridge a(c)2 5 cm segmental bone defects of left radius in 15 rhesus monkeys as experimental group, bio derived materials only were implanted to bridge same size defects of right radius as control group. Every 3 monkeys were sacrificed in the 1st, the 2nd, the 3rd, the 6th and the 12th weeks postoperatively and the expressions of IL 2 and IL 2 receptor in blood and graft samples were detected quantitatively by enzyme linked immuneosorbent assay (ELISA). Results There was no significant difference in the contents of IL 2 and its receptor between 2 groups(?P?￡3/40.05￡(c). The contents of IL 2 and its receptor increased from the 2nd week and maintained high level from the 2nd to the 6th week, but decreased after 6 weeks. Conclusion Tissue engineered bones constructed with allogeneic MSCs and bio derived materials show low immunogenicity. Allogeneic MSCs may be used as seed cells to construct tissue engineered bone.a$?3/4

关 键 词：组织工程骨 植入 IL-2R 异体 生物衍生骨 猕猴 白细胞介素2 骨材料 表达 种子细胞 

分 类 号：R318.1[医药卫生—生物医学工程]