士的宁分子印迹整体柱的制备  

Preparation of Strychnine Molecularly Imprinted Monolithic Column

作　　者：张静[1] 贺浪冲[1] 傅强[1] 

机构地区：[1]西安交通大学医学院,西安710061

出　　处：《分析化学》2005年第1期113-116,共4页Chinese Journal of Analytical Chemistry

摘　　要：以士的宁为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯作为交联剂,甲苯和十二醇混合溶液为致孔剂,用原位分子印迹技术,合成了士的宁分子印迹整体柱。通过优化合成条件,结果显示:模板分子、功能单体与交联剂之间的比例以 1∶4∶16最佳,致孔剂中甲苯的最佳含量为 18% (V/V);对士的宁整体柱的色谱条件包括流动相组成、流速、柱温等进行了考察,并用于士的宁和马钱子碱的分离,其分离因子为 3 5。Using strychnine as the template, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate ( EDMA) as the cross-linker, a strychnine imprinted monolithic column was prepared for the separation of the strychnine and brucine by in-situ, molecular imprinted technique. Then, the synthesis condition was optimized. The result showed the best ratio of template, functional monomer and cross-link was 1:4:16, and the toluene content in porogen was 18%. The effects of mobile phase composition, flow rate and column temperature on retention and separation factor were investigated. The maximum separation factor between strychnine and brucine was 3.5.

关 键 词：整体柱 分子印迹 模板分子 致孔剂 功能单体 因子 二甲基 士的宁 马钱子碱 色谱条件 

分 类 号：D919[医药卫生—法医学]