间接原子吸收法测定葡萄籽提取物中的原花青素  

Atomic Absorption Spectrometric Method for Indirect Determination of Oligomeric Procyanidins in Grape Seeds

作　　者：马亚军[1] 郎惠云[1] 董发昕[1] 

机构地区：[1]西北大学化学系

出　　处：《分析化学》2005年第1期120-122,共3页Chinese Journal of Analytical Chemistry

摘　　要：提出了间接测定葡萄籽提取物中原花青素的原子吸收光谱法.它是基于原花青素能与醋酸铜发生络合反应,生成难溶于水的棕黄色沉淀,经离心分离后,用原子吸收法测定上清液中过量的铜离子,可间接测定原花青素的含量.方法线性范围为3.0～30.0 mg/L;RSD为1.1%～1.2 %;回收率为98.9%～102.0%.A new method of determining oligomeric procyanidins by atomic absorption spectrometry (AAS) was studied. The method is based on the reaction of oligomeric procyanidins with Cu2+ and lead to yellow precipitate. The precipitate was centrifuged to subside. The excess Cu2+ in supernatant was measured by AAS, and the concentration of oligomeric procyanidins could be determined indirectly. The linear region was 3.0 similar to 30.0 mg/L, RSD = 1.1% similar to 1.2 %, and the recovery is 98.9% similar to 102.0%, The method has been applied to determine oligomeric procyanidins with good result.

关 键 词：原花青素 葡萄籽提取物 醋酸铜 RSD 含量 过量 上清液 间接原子吸收法 法线 间接测定 

分 类 号：O629[理学—有机化学] O657.3[理学—化学]