川芎提取液动物血药浓度测定及其对钙激活钾通道作用初探  

Determination of Serum Concentrations of Chuanxiong Extract and Its Action on Ca^(2+)-activated K Channel in Animals

作　　者：叶云[1] 杨艳[2] 冯碧敏[1] 张昊[1] 杨艳艳[2] 

机构地区：[1]四川泸州医学院附属医院药剂科,泸州市646000 [2]四川泸州医学院心肌电生理学研究室,泸州市646000

出　　处：《中国药房》2005年第1期19-22,共4页China Pharmacy

基　　金：四川省教育厅自然科学科技项目 (2003A058)

摘　　要：目的 :尝试建立电生理与血清药理学技术相结合的方法用于川芎提取液扩张冠状动脉机制的研究。方法 :对大、小鼠灌胃给予川芎提取液后 ,用高效液相色谱(HPLC)法测定鼠血清中川芎嗪(TMP)的含量 ,为电生理实验提供依据 ;用膜片钳方法研究川芎提取液对猪冠状动脉平滑肌钙激活钾通道的作用。结果 :TMP血清浓度线性范围为0 216～54μg/ml(r=0 9999) ,回收率为100 7%～102 4% ,大、小鼠血药浓度分别为 (3 56±3 01)、(8 19±6 57) μg/ml;膜片钳实验结果表明鼠含药鼠血清对猪平滑肌上钙激活钾通道没有显著激活作用。结论 :本文建立的测定TPM含量的方法适用、可靠 ;应选用高质量药材或提高药物的生物利用度才有望完善电生理与血清药理学技术相结合的方法。OBJECTIVE:To try to establish a combined serum-pharmacology and electrophysiology method for the study of mechanism of chuanxiong extract in dilatating coronary artery.METHODS:Chuanxiong extract was intragastrically given to rats and mice,then the serum contents of tetramethylpyrazine(TMP)were determined by HPLC,which provided data for electrophysiological study.The action of chuanxiong extract on Ca 2+ -activated K channel(Kca)of smooth muscle of coronary artery in pigs was studied by patch clamp technique.RESULTS:The calibration curve of TMP was linear in the range of0.216～54μg/ml(r=0.9999).The recovery was100.7%～102.4%.The serum concentrations of TMP in rats and mice were(3.56±3.01)and(8.19±6.57)μg/ml respectively.The chuanxiong extract could not markedly activate the Kca of smooth muscle of coronary artery in patch clamp study.CONCLUSION:The established method for TMP determination is feasible and relieable.In order to improve the combined electrophysiology and serum pharmacology technique,high quality medical materials should be adopted and the bioavailability improved.

关 键 词：川芎 川芎嗪 高效液相色谱法 血清药理学 膜片钳技术 

分 类 号：R285.5[医药卫生—中药学] R965[医药卫生—中医学]