神经细胞黏附分子样蛋白对小鼠NK细胞活性的影响  

Influence of Neural Cell Adhesion Molecule-like Protein (NCAML) on Activity of Murine NK Cell

作　　者：刘青[1] 王宣军[2] 吴秀丽[3] 王丽颖[3] 于永利[3] 

机构地区：[1]吉林大学第一医院,长春130021 [2]长春生物制品研究所,长春130062 [3]吉林大学基础医学院,长春130021

出　　处：《中国生物制品学杂志》2005年第1期23-25,共3页Chinese Journal of Biologicals

基　　金：国家自然科学基金资助 (批准号 3 0 0 70 70 5 )

摘　　要：目的　研究神经细胞黏附分子样蛋白对小鼠NK细胞活性的影响。方法　将神经细胞粘附分子样蛋白的编码基因连接在补体C3d基因的下游 ,构建融合基因 ,再将此融合基因克隆入真核细胞表达质粒。用此重组质粒免疫小鼠 ,检测小鼠体内的抗体和NK细胞的活性。结果　小鼠体内产生了神经细胞粘附分子样蛋白的特异性抗体 ,而且小鼠脾细胞的NK活性增强。结论　神经细胞粘附分子样蛋白可能具有抑制NK细胞的活性 ,在被特异性抗体识别和结合后 ,这种抑制作用减弱或消失。Objective To study the influence of neural cell adhesion molecule-like protein (NCAML) on the activity of murine NK cell.Methods Insert the gene encoding NCAML downstream to C3d gene of complement and clone the constructed recombinant plasmid into eukaryotic cell expression vector pcDNA3. Immunize mice with the recombinant plasmid pcDNA3-NCAML/3C3d and detect the in vivo antibody and NK cell activities of the mice.Results The specific antibody to NCAML was induced in the mice, and the NK activity of murine splenocyte was enhanced.Conclusion NCAML might inhibit the activity of NK cell. However, after NCAML was recognized by and bound to its specific antibody, the inhibitory effect decreased or disappeared.

关 键 词：小鼠 蛋白 NK细胞活性 神经细胞粘附分子 体内 特异性抗体 神经细胞黏附分子 补体C3d 克隆 重组质粒 

分 类 号：Q78[生物学—分子生物学] R285.5[医药卫生—中药学]