环孢素A与盐酸小檗碱联用预防大鼠心脏移植排斥反应  

Prevention of acute heart allograft rejection by berberine combined with cyclosporine A in rats

作　　者：倪兴华[1] 曾耀英[1] 狄静芳[1] 何贤辉[1] 肇静娴[1] 林羿[2] 王子栋[3] 曾山[1] 

机构地区：[1]暨南大学组织移植与免疫教育部重点实验室 [2]中山大学医学院组胚教研室 [3]暨南大学医学院生理教研室

出　　处：《中华器官移植杂志》2005年第1期53-54,共2页Chinese Journal of Organ Transplantation

基　　金：国家重点基础研究发展"973"项目基金资助(G1999054303);国家自然科学基金重点项目基金资助(No.39930230);广东省"十五"科技计划重大专项基金资助(A302020204)

摘　　要：目的探讨环孢素A(CsA)与盐酸小檗碱联用对同种异基因大鼠心脏移植排斥反应的抑制效果。方法建立Wistar大鼠心脏到SD大鼠的异位(腹腔内)心脏移植模型,术后单独使用CsA、盐酸小檗碱以及联合使用CsA和盐酸小檗碱进行免疫抑制治疗,观察各组移植心脏的存活时间。结果单独使用不同剂量CsA者移植心脏的存活时间较安慰剂组明显延长(P<0.01);CsA和盐酸小檗碱联用组移植心脏的存活时间较单用小剂量CsA组明显延长(P<0.01);单用盐酸小檗碱组与安慰剂组相比,移植心脏存活时间的差异无统计学意义(P>0.05)。结论CsA与盐酸小檗碱联用预防大鼠心脏移植后的排斥反应具有协同作用。Objective To investigate the effect of Cyclosporine A in combination with Berberine to induce immune tolerance in rats of allogenic cardiac transplantation. Methods The rats’heterotopic cardiac transplantation model was established by Ono’s method. After heterotopic cardiac transplantation, CsA alone, Berberine alone or CsA plus Berberine were used for immunosuppressive therapy. The cardiac allograft survival and levels of BUN and Cr in the recipients’plasma were measured. ~Results In CsA-treated group, the survival time of the cardiac allografts was significantly longer than that in placebo group (P<~0.01 ). In the CsA plus Berberine group, the survival time of the cardiac ~allografts was significantly longer than that in low dose of CsA-treated group (P<~0.01 ). There was no significant difference in the survival time of the cardiac grafts between berberine group and placebo group (P>~0.05 ). Conclusion Combined use of CsA with Berberine can produce synergistic effects in prevention of acute cardiac rejection after heart transplantation in rats.

关 键 词：小檗碱 环孢菌素 药物协同作用 移植物排斥 心脏移植 

分 类 号：R96[医药卫生—药理学]