斑秃患者皮损处T淋巴细胞表型的研究及Fas的表达  

A study on infiltrating T-lymphocytic phenotype and Fas expression in skin lesion area of pelade patients

作　　者：陈丽芳[1] 史维平[1] 刘金华[1] 陈小敏[1] 秦小卫[1] 郝树媛[1] 郑杏改[1] 尚玉兰[1] 王宇芳[1] 

机构地区：[1]山西省人民医院,030012

出　　处：《山西医药杂志》2005年第1期9-11,i001,共4页Shanxi Medical Journal

基　　金：山西省卫生厅科研基金资助项目 (2 0 0 132 )

摘　　要：目的　研究斑秃患者皮损处浸润T淋巴细胞的表型及Fas的表达情况。方法　应用免疫组织化学技术检测斑秃患者皮损处CD4和CD8T淋巴细胞及Fas的表达。结果　CD4和CD8:活动期和稳定期斑秃患者皮损处无论是毛囊周围还是血管周围CD4T淋巴细胞浸润数量均明显多于CD8T淋巴细胞 (P <0 .0 5 ) ;斑秃活动期的CD4和CD8T淋巴细胞浸润数量与稳定期和对照组相比较差异有统计学意义 (P <0 .0 5 )。Fas :与对照组相比较 ,斑秃患者皮损处毛囊角质形成细胞Fas的表达例数较多 (P <0 .0 5 )。结论　斑秃患者皮损处浸润T淋巴细胞以CD4T细胞占优势 ;Fas在斑秃皮损处毛囊角质形成细胞异常表达。Objective To investigate the phenotype of infiltrating T lymphocytes and the expression of Fas protein in the skin lesion areas of alopecia areata (AA) patients.Methods The sections of skin from the lesion areas of active and inactive AA patients were stained immunohistochemically to observe the infiltration of T-lymphocytes and the expression of Fas protein.Results CD4 and CD8 lymphocytes:in the skin lesion areas of both active and inactive AA patients, there were more CD4 T-lymphocytes than CD8 T-lymphocytes around blood vessels and hair follicles (P<0.05). In the skin lesion areas of active AA patients, the number of CD4 and CD8 lymphocytes was significantly increased when compared with inactive AA patients or normal human subjects.Fas protein:when compared with normal human subjects, there were more keratinocyte cells that expressed Fas protein in hair follicles of AA patients (P<0.05).Conclusion The CD4 T-lymphocytes are predominant over CD8 T-lymphocytes in the skin lesion areas of AA patients.The expression of Fas protein on the plasma membrane and in the cytoplasma of keratinocyte cells is abnormally increased in the areas.

关 键 词：斑秃 患者 皮损 T淋巴细胞 表达 CD8 CD4 表型 数量 毛囊 

分 类 号：R758.71[医药卫生—皮肤病学与性病学] R275.987.1[医药卫生—临床医学]