NS-398诱导肝癌细胞凋亡的实验研究  

Experimental study on apoptosis of HepG_2 cell induced by NS 398Experimental study on apoptosis of HepG 2 cell induced by NS-398

作　　者：张岭漪[1] 张有成[1] 傅生军[1] 王祥[1] 马力[1] 

机构地区：[1]兰州大学第二医院消化内科

出　　处：《中国临床药理学与治疗学》2005年第1期61-64,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：甘肃省教育厅科研资助项目 (№ 2 0 0 3 0 3 3 0 6)

摘　　要：目的 :研究选择性环氧化酶 2 (COX 2 )抑制剂NS 398对人肝癌细胞HepG2 凋亡的影响 ,及其相关蛋白Bcl 2在细胞凋亡中的作用。方法 :通过体外细胞培养 ,应用荧光显微镜、透射电镜、流式细胞仪观察NS 398对HepG2 的凋亡诱导作用 ,应用免疫细胞化学法观察Bcl 2在人肝癌细胞HepG2 凋亡中的表达。结果 :NS 398处理HepG2 细胞后 ,电镜下可见到细胞核固缩、染色质凝集成新月型紧靠核膜周边 ,核碎裂、染色质片断化等典型的细胞凋亡形态变化。荧光显微镜及流式细胞检测则未见凋亡征象。免疫细胞化学分析显示 ,NS 398处理后的HepG2 细胞 ,其Bcl 2表达较对照组明显下降。结论 :COX 2选择性抑制剂NS 398对人肝癌HepG2 细胞有显著的凋亡诱导作用 ;抗凋亡基因Bcl 2在NS 398诱导的HepG2 细胞凋亡中有重要的调控作用。AIM : To study the effects of selective COX 2 inhibitor NS 398 on apoptosis of HepG 2 cell line and to evaluate the modulation activity of Bcl 2 on the procession of HepG 2 cell apoptosis. METHODS : HepG 2 cells were cultured in RPMI1640 media and treated by NS 398 for 24, 48 and 72 hours. The apoptosis of HepG 2 cells was observed by fluorescent microscopy, transmission electron microscopy and flow cytometric analysis. The expression of Bcl 2 in HepG 2 cells during the apoptosis was measured by immunocellularchemical staining. RESULTS : NS 398 obviously induced some morphologic features of HepG 2 cell apoptosis such as cellular nucleus shrinking, nucleus fragments, luniform chromatin agglutinating and chromatin fragmentation. Compared with the control group, the NS 398 group significantly decreased the expression of Bcl 2. CONCLUSION : COX 2 selective inhibitor NS 398 can significantly induce the apoptosis of hepG 2 cells, and Bcl 2 may play an important role in modulating apoptosis induced by NS 398.

关 键 词：肝细胞癌 环氧化酶-2抑制剂 NS-398 凋亡 BCL-2 

分 类 号：R966[医药卫生—药理学]