猪生殖与呼吸综合征ORF5基因疫苗的构建及其安全性和免疫原性检测  

Construction of ORF5 gene vaccine against PRRSV and detection of its safety and immunogenicity

作　　者：程安春[1] 汪铭书[1] 希尼尼根[2] 豆文波[2] 陈希文[2] 陈孝跃[1] 

机构地区：[1]动物疫病与人类健康四川省重点实验室,四川雅安625014 [2]四川农业大学动物科技学院,四川雅安625014

出　　处：《中国兽医科技》2005年第1期27-35,共9页Chinese Journal of Veterinary Science and Technology

基　　金：国家科技攻关重大项目(2004BA901A03);四川省生物技术项目(01NG018 03);四川省重点建设学科项目(SZD0418)

摘　　要：克隆了猪生殖与呼吸综合征病毒(PRRSV)SC2株ORF5基因。测序分析表明SC2株属美洲型PRRSV。构建的ORF5基因疫苗经脂质体转染Marc 145细胞后,第26h检测到疫苗蛋白的特异性表达,第56h达到高峰。疫苗肌肉注射小鼠和仔猪后能迅速分布到各组织器官中,未发现核酸疫苗与宿主细胞染色体整合现象;且能使仔猪产生体液和细胞免疫应答,免疫期持续135d以上。结果表明,构建的PRRSVORF5基因疫苗具有良好的安全性和免疫原性。ORF5 cDNA cloning and sequencing analysis of porcine reproductive and respiratory syndromevirus (PRRSV) SC2 strain showed that the strain belonged to PRRSV American type. The constructed ORF5 gene vaccinewas transferred into Marc-145 cell line. Intense fluorescence could be observed at 26th hour post-transfection(PT) in the Marc-145 cells and the amount of fluorescence peaked at 56th hour PT. The ORF5 gene could be detected by PCR from tissues and organs of mice and pigs immunized with the ORF5 gene vaccineby intramuscular injection. The ORF5 gene was not integrated into the chromosomes of the animal. Humoral and cellular immuneresponses were obtained in pigs by intramuscular injectionand gene gun transferroutes and persisted for more than 135 days. The results indicated that the ORF5 gene vaccineis immunogenicand safety.

关 键 词：猪生殖与呼吸综合征病毒 ORF5基因 基因疫苗 安全性 免疫原性 

分 类 号：S852.651[农业科学—基础兽医学]