抗鸡传染性法氏囊病毒单克隆抗体的定量检测  

THE QUANTITATIVE DETECTIVING OF MONOCLONAL ANTIBODY TO INFECTIOUS BURSAL DISEASE VIRUS

作　　者：陆苹[1] 倪学英 胡志贤[1] 何云海 王国丰[1] 

机构地区：[1]上海农学院生物技术研究所,上海201101

出　　处：《上海农学院学报》1993年第4期261-265,共5页

基　　金：本课题为上海市科委科学技术发展基金项目

摘　　要：以免抗鼠 IgG 作为抗血清,首先验证了应用单向琼脂扩散法与酶联兔疫测法(ELISA)测定中试样品中抗鸡传染性法氏囊病毒(IBDV)单抗浓度的相应关系;以 DEAE 纤维离子交换层析法纯化的鼠 IgG(3.8mg/ml)作标准样品,以单向琼扩法确定中试样品 F.中具有生物活性单抗含量为330uG/ml,并以 F.作为 ELISA 标准样品,作标准曲线,检测不同样品中抗 IBDV 单抗含量皆在50ug/ml(此为1:10稀稀应用的浓度标准值)以上,ELISA 效价皆在10^(-4)以上。证明了我们的中试产品 OD 值在0.050以上,都可以1:10稀释,可靠地投放市场广泛应用。We identified the relationship between the results of singlc dimcntional Immunodiffusion Test in Agar(SDIDA)and ELISA.Firstly,we determined the concentration of McAb in F sample was 330ug/ml using ELISA test,according to the standard sample which is mice IgG (3.8mg/ml)purified by DEAE-Cellulose Columm Chromatography methods.Then,we get a standard curve of F_0sample as ELISA standard sample.The concentrations of different groups of McAb sample to IBDV were all above 50ug/ml(the standard concentration to use)and the titer of ELISA were all above 10~4.The all of experiments proved that the middle trial production can be used widespread and assurelly after 1:10 dillutor.

关 键 词：单克隆抗体 IBD 鸡病 检测 

分 类 号：S858.315.3[农业科学—临床兽医学]