EMS对甘蓝型油菜离体小孢子胚胎发生能力的影响  

Effect of EMS treatment on microspore embryogenesis in vitro in Brassica napus

作　　者：和江明[1] 王敬乔[1] 陈薇[1] 李根泽[1] 寸守铣[1] 

机构地区：[1]云南省农业科学院生物技术研究所,云南昆明650223

出　　处：《西南农业学报》2004年第6期690-693,共4页Southwest China Journal of Agricultural Sciences

基　　金：云南省应用基础研究基金资助项目(2001C0033Q)

摘　　要：EMS(甲基磺酸乙酯)是烈性致癌物质,对育种者而言,它又是很好的诱变剂。本试验用不同的EMS浓度(0,1 0,1 5,2 0,2 5,3 0mmol/L)和不同时间(12,24,36h)处理甘蓝型油菜的离体小孢子,通过统计每花蕾产胚的数量,观察到随着EMS浓度的增高和处理时间的延长,每花蕾产胚的数量减少,即小孢子的胚胎发生能力减弱。研究得出当EMS浓度为2 5mmol/L,处理时间为24h,每花蕾产胚的数量接近对照产胚数量的一半,根据半致死剂量(LC50)原则,这一浓度和时间对EMS诱变甘蓝型油菜离体小孢子具有重要的应用价值。Although EMS was a strong cancerogen, it was a good mutagen for breeder. The microspores of Brassica napus in vitro were treated by mutagen ethyl methane sulphonate (EMS) at different doses (0,1.0,1.5,2.0,2.5,3.0 mmol·L^(-1)) and different duration (12, 24, 36 h).The rerults showed the number of every alabastrum producing embryos decreased,that was the microspore embryogenesis decreased, with the increase of EMS doses and duration of EMS treatment.Every alabastrum producing embryos was about half of the CK when EMS dose was 2.5 mmol·L^(-1) and the duration of EMS treatment was 24 h.According to medial lethal concentration(LC_(50)), this EMS dose and duration had important applied value on microspore mutagenesis in vitro in Brassica napus by EMS treatment.

关 键 词：EMS 油菜 小孢子 胚胎发生 

分 类 号：S634.2[农业科学—蔬菜学]