含Calbindin-D28K mRNA的神经元在大鼠脑干中的分布—原位杂交组织化学法观察  

LOCALIZATION OF THE NEURONS CONTAINING CALBINDIN-D_(28K) mRNA IN THE RAT BRAINSTEM

作　　者：张纪岩[1] 张建华[1] 

机构地区：[1]第四军医大学解剖学教研室

出　　处：《神经解剖学杂志》1993年第2期207-211,共5页Chinese Journal of Neuroanatomy

摘　　要：用原位杂交组织化学技术,对含Calbindin-D28K mRNA的神经元在大鼠脑干中的分布进行全了面观察。发现不同脑区或核团中所含阳性神经元的数量及其标记强度各不相同。其中含强阳性杂交信号的区域有:小脑皮质、外侧丘系核、斜方体核、下橄榄核及臂旁核;含中等强度杂交信号的区域包括:脚间核、黑质致密部、耳蜗核和最后区。而在其它大部分脑区中杂交信号呈弱阳性,如:上丘、顶盖前区、红核、三叉神经感觉核簇、后索核、下丘、前庭核群、孤束核、中央灰质和网状结构中的部分核团。含Calbindin-D28KmRNA的神经元在脑干中的这种区域特异性分布特点提示在神经系统的某些生理功能中,Calbindin-D28K可能起重要的作用。Using in situ hybridization histochemistry, we examined the localization of the neurons containing calbindin-D_(28K) mRNA in the rat brainstem. In some regions, many neurons were strongly labeled, such as: the cerebellar cortex, the nucleus of lateral lemniseus, the nucleus of trapezoid body) the inferior olivary nucleus and the parabrachial nucleus, However, other regions such as the interpeduncular nucleus, the substantia nigra (compact part), the cochlear nucleus, and the area postrema contained neurons labeled moderately. The regions containing weakly labeled neurons were as follows: the superior colliculus, the pretectal region, the red nucleus, the principal and the spinal nuclei of trigeminal nerve, the gracileand cuneate nuclei, the inferior collicultis, the vestibular nuclei, the nucleus of the solitary- tract and some nuclei of the reticular formation. Therefore, the neurons containing calbindin-D_(28K) mRNA showed a regionspecific distribution in rat brainstem, suggesting an important role of calbindin-D_(28K) in some brain functions.

关 键 词：CALBINDIN MRNA 脑干 蛋白 神经元 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]