人参皂甙对小脑生长锥影响的体外培养观察  

GINSENG SAPONINS: IN VITRO EFFECT ON GROWTH CONE OF NEWBORN MOUSE CEREBELLA

作　　者：郭诗玫[1] 郝正玲 张素春[2] 沈馨亚[2] 

机构地区：[1]上海医科大学肿瘤医院药剂科 [2]上海医科大学解剖学教研室

出　　处：《沈阳药学院学报》1993年第1期47-50,共4页

基　　金：自然科学基金No.3880330资助

摘　　要：自吉林人参根部制备的总皂甙(Rx),按每毫升培养液含50,100,200μg的浓度,对新生(P-O)B_6C_3小鼠的小脑进行Maximow器官——组织型培养,总数达120片。在体外培养第2,3,5天分别在相差显微镜下进行活体观察,对生长锥的长度和密度作进一步的观察和测量,同时结合神经染色和扫描电镜的观察与记录,结果显示实验组在生长锥的生长趋势较对照组明显,尤以100μg/ml组最为显著,具有统计学意义。本研究结果提示人参皂甙对神经组织的生长和神经网络的建立具有促进作用。Ginsenoside (R_x) of ginseng root was added to the culture medium in the following different concentrations: 50, 100 and 200 μg in 1 ml. of the regular medium. The control group was spared ginsenoside. The primary organotypical culture of newborn (p-o) mouse cerebella was carried out as usual (Shen, 1985, 1988). No antibiotics was used. The total cultured explants were 120 in number. The proliferative effects of neurites in vitro were consecutively observed on the 2nd, the 3rd, and the 5tb day under phasecontrast microscope and some of them were stained with toludine blue and fixed for scanning electron microscopy. The evaluation of their activity was based on the neurite length produced by the explant. We found that the growth cones in the 100 μg/ml group were obviously enhanced when compared with those in other groups. The results suggest that ginsenosides have a promoting effect on neurite extension.

关 键 词：人参皂甙 神经培养 小脑 生长锥 

分 类 号：R285.5[医药卫生—中药学]