胰多肽对大鼠垂体前叶β-内啡肽和催乳素释放的影响  

PANCREATIC POLYPEPTIDE IN THE CONTROL OF β-ENDORPHIN AND PROLACTIN RELEASE FROM RAT ANTERIOR PITUITARY IN VIVO AND IN VITRO

作　　者：许荣焜[1] 黄曼影[1] 韩小四[1] 郭传海[1] 周远征[1] 黄醒亚[1] 

机构地区：[1]中国医学科学院基础医学研究所中国协和医科大学基础医学部生理研究室,北京100005

出　　处：《生理学报》1993年第3期215-222,共8页Acta Physiologica Sinica

基　　金：国家自然科学基金(No.38970313)

摘　　要：本工作分在体和离体两部分实验。(1)给清醒自由活动的大鼠第三脑室内微量注射0.5和2.0μg的胰多肽(PP),观察到PP显著抑制前叶垂体β-内啡肽(β-EP)和催乳素(PRL)的静息分泌,抑制效应随PP的剂量增加而增大。此外,0.5μgPP能部分抑制限制性应激引起的PRL的释放;2.0μgPP只部分抑制此应激引起的β-EP的释放,并显著抑制应激时PRL的释放。(2)离体实验于体外培养的前叶垂体细胞中,分别加入0.05,0.625.1.00μgPP,一起孵育1h,看到0.625和1.00μg的PP显著抑制PTL的释放,抑制效应依PP的剂量增加而增大;上述三种剂量的PP对前叶垂体细胞β-EP的分泌均无影响。这些结果提示,PP可能通过某种下丘脑机制抑制前叶垂体β-EP和PRL的释放,也可直接作用于前叶垂体细胞抑制PRL的释放。This study was designed to examine the possible effects of pancreatic polypeptide(PP) on β-endorphin (β-EP) and prolactin (PRL) release from rat anterior pituitary inrivo and in ritro. Injection of 0.5 μg or 2.0 μg PP into the third ventricle of the brain(3rd v. i.) produced a significant decrease of the β-EP and PRL resting secretion. 0.5μg PP (3rd v. i.) did not affect restraint stress--induced release of β-EP, but partiallylowered stress induced release of PRL. 2.0 μg PP (3rd v. i.) partially reduced re-straint stress--induced release of β-EP and completely suppressed stress--induced releaseof PRL. In order to investigate a possible direct action of PP on β-EP and PRL secre-tion from the anterior pituitary gland. we incubated dispersed anterior pituitary cellswith synthetic PP (0.05, 0.625 and 1.00 μg) for 1 n, the secretion of β--EP was notaffected at any dosage tested, but 0.625 and 1.00 μg PP significantly decreased thePRL secretion. These data indicate that PP may have an inhibitory role in the controlof β--EP secretion at the level of the hypothalamus, and an inhibitory role in the controlof PRL secretion at the level of either hypothalamus or anterior pituitary.

关 键 词：胰多肽 催乳素 Β-内啡肽 垂体 

分 类 号：R335.1[医药卫生—人体生理学]