大鼠再生肝刺激因子抗四氯化碳损伤的研究  

A STUDY OF REGENERATING LIVER PROTECTS AGAINST CARBON TETRACHLORIDE INJURY IN RAT

作　　者：宫德正[1] 邵青[1] 梅懋华[1] 

机构地区：[1]大连医学院生理教研室,大连116023

出　　处：《生理学报》1993年第5期498-504,共7页Acta Physiologica Sinica

基　　金：辽宁省教委资助课题

摘　　要：我们以往的研究证明，大鼠再生肝具有抗四氯化碳损伤的能力。本工作进一步研究其机制，首先从部分（６８％）肝切除后不同的再生肝的取肝刺激因子，用＾３Ｈ胸腺嘧喧核苷测定ｒＨＳＳ的生物活性，结果表明部分切除肝后７２ｈ的ｒＨＳＳ活性较对照组约增加７．７倍。然后将ｒＨＳＳ注射给小鼠，观察其抗ＣＣｌ４损伤肝的效应。In our previous study we had demonstrated that regenerating liver of rats had anability to resist CCl_4 injury. In this paper, the underlytng mechanism was furtherinbestigated. Hepatic stimulator substance from regenerating liver (rHSS) at differenttime after partial (68%) hepatectomy was extracted and assaied for its biological ac-tivity by using ~3H-thymidine. The activity is approximately seven--fold as comparedwith the control. Then, rHSS was given to rats to observe its effect against CCl_4 in-jury both in vivo and in vitro. The results were as follows: rHSS decreases the mortali--ty of CCl_4 intoxicated rats and suppresses the elevation of serum alanine aminotrans--ferase (ALT) and aspartate aminotransferase (AST) induced by CCl_4 in vivo. AlsorHSS in creases the cell viability and decreases the leakage of intracellular ALT of hep--atocytes poisoning by CCl_4. The above mentioned results suggest rHSS is an importantmechanism for regenerating liver to protect against CCl_4 poisoning.

关 键 词：再生 肝刺激因子 四氯化碳 肝切除 

分 类 号：R657.3[医药卫生—外科学]