甜菜坏死黄脉病毒外壳蛋白基因的克隆和序列分析  

The Cloning and Sequencing of Coat Protein Gene from Beet Necrotic Yellow Vein Virus

作　　者：姚华建[1] 刘仪[1] 于嘉林[1] 蔡祝南[1] 敖光明[1] 杨莉莉[1] 

机构地区：[1]北京农业大学国家农业生物技术重点实验室

出　　处：《生物工程学报》1993年第2期147-151,共5页Chinese Journal of Biotechnology

摘　　要：以甜菜坏死黄脉病毒(BNYVV)内蒙分离物的RNA为模板,通过PCR扩增获得外壳蛋白(CP)基因的目的片段。将其重组到pGEM-7zf(+)并转化JM101得到了含有完整CP基因的重组子。采用双脱氧终止法进行序列分析,结果表明CP基因为567 nt,与文献[1]报道相比,氨基酸和核苷酸的同源性分别为98.4%和96.7%。The beet necrotic yellow vein virus (BNYVV) isolate NM was isolated from sugarbeet infected with rhizomania in Inner Mongolia of China. The cDNA of BNYVV coat protein (CP) gene was amplified from the extracted RNA of BNYVV isolate NM by using the polymerase chain reaction (PCR), and cloned into pGEM-7zf(+). Its complete nucleotide sequence has been determined by means of Sanger's dideoxy-mediated cbain-termination method. The result shows that BNYVV isolate NM CP gene has 567nt.It shares 98.4% and 96.7% identity with CP gene of isolate F_(13) in terms of amino acid and nucleotide sequence respectively.

关 键 词：甜菜 坏死黄脉病毒 外壳蛋白基因 

分 类 号：S188[农业科学—农业基础科学]