国产生物反应器及其在病毒培养中的应用  

A Domestic Cell Bioreactor and its Application in Viruses Culture

作　　者：董树沛 顾小华[1] 陈因良[1] 严春 姜壁贵 赵一鸣 宋家骊 陈列胜 陈文兰 

机构地区：[1]华东化工学院生化工程研究所,上海 200237 [2]卫生部上海生物制品研究所

出　　处：《生物工程学报》1993年第2期137-141,共5页Chinese Journal of Biotechnology

摘　　要：本文简要介绍了华东化工学院研制的CellCul-20细胞培养生物反应器,报道了此反应器用于细胞和病毒培养的情况。经严格的无菌试验和一年多的培养表明:CellCul-20反应器具有优良的抗污染性能,其主要参数的控制完全满足细胞培养的要求,并能根据培养过程的实际情况,随时对参数进行修正。用所开发的培养工艺进行Vero细胞和乙脑病毒培养,细胞在较短时间内达到高密度,乙脑病毒滴度和抗原效价均取得了较为满意的结果。在国际上第一次成功地应用vero细胞大规模培养乙脑病毒原制苗。The cell culture bioreactor (CellCul-20) and its application in cells and viruses culture are described in this paper. It was proved by aseptic test and one-year's operation that CellCul-20 can keep aseptic after being autoclaved and control or adjust the main parameters in the set range. A bigh cell density and virus titre were reached when culturing Vero cells and Japanese encepbalitis virus (JEV) in this cell bioreactor. It's the first report about large scale culture of JEV-infected Vero cells to prepare primary JEV vaccine. A series of suggestion are proposed for the improvement of CellCul-20.

关 键 词：生物反应器 细胞培养 病毒 

分 类 号：Q81[生物学—生物工程]