转化脂介导小麦原生质体转化及转基因白化苗的再生  

Lipofectin Mediated Transformation of Wheat Protoplasts and Regeneration of Transgenic Albino Plantlets

作　　者：朱祯[1] 孙宝林 刘春明[1] 肖桂芳[1] 李向辉[1] 

机构地区：[1]中国科学院遗传研究所

出　　处：《生物工程学报》1993年第4期320-323,共4页Chinese Journal of Biotechnology

摘　　要：应用转化脂介导转化技术成功地将含有潮霉素磷酸转移酶基因的Ｅ．ｃｏｌｉ质粒ｐＣＧＮ１０５５导入到了小麦（徐州２１１）原生质体内，并由此获得了转化愈伤组织。根据Ｈｐｔ抗生及ＤＮＡ分子杂交证实转化频率高达６．０－８．８％，在分化培养基上转化愈伤组织再生出了白化植株，通过Ｓｏｕｔｈｅｒｎ杂交和酶活性检测证明外源Ｈｐｔ基因存在于转化植株（白化苗）内，并进行了有效的表达。E. coli plasmid pCGN1055 containing hygromycin phosphotransferase (Hpt) gene under control of plant expression promoter was introduced into wheat protoplasts by lipofectin-mediated transformation, and transgenic albino plantlets were obtained, which were confirmed by assay of Hpt activity and Southern blot. The transformation frequencies were 6.0% and 8.8% according to hygromycin resistance and DNA molecular hybridization (Dotblot). The results indicated the foreign gene existed and expressed in transgenic plantlets.

关 键 词：小麦 原生质体 转化脂 转基因植物 

分 类 号：S188[农业科学—农业基础科学]