PCR 扩增人型结核杆菌 DNA 及结核杆菌属 DNA 探针的制备  

The Amplification of Mycobacterium Tuberculosis DNA by PCR and The Preparation of DNA Probe of Mycobacterium Tuberculosis

作　　者：丁晓华[1] 杨平[1] 刘薇茜[1] 谭云[1] 赵文先[1] 蔡建斌 梅国华 潘慧可 

机构地区：[1]湖北医学院病毒研究所,武汉430071 [2]武汉钢铁公司第二职工医院,武汉430085

出　　处：《生物化学与生物物理进展》1993年第3期195-198,共4页Progress In Biochemistry and Biophysics

摘　　要：用多聚酶链反应(PCR)方法扩增人型、牛型结核杆菌基因组 DNA,获得特异的158 bpDNA 片段,而从另外十三种分枝杆菌未见到特异的扩增产物.回收158 bpDNA 片段作探针,它除与人型、牛型结核杆菌有特异的杂交信号外,与金黄色葡萄球菌、绿脓杆菌及一些分枝杆菌皆没有杂交反应.结果表明,PCR 可用于检测结核杆菌基因组 DNA,扩增产物158 bp DNA 片段可作为探针用于检测人型、牛型结核杆菌并鉴别结核杆菌与其它分枝杆菌.The DNA of Mycobacterium tuberculosis is amplified by polymerase chain reaction and we get a spe-cific 158 bp DNA fragment of Mycobacterium tuberculosis.The DNA of Mycobacterium bovis is also am-plified,but Other thirteen mycobacteria are not.The DNA fragment is recovered and used as a DNAprobe,It can hybridizes with DNA of Mycobacterium tuberculosis and Mycobacterium bovis,but not withthe DNA of Staphylococcus aureus,Bacillus pyocyaneus and other mycobacteria.This result suggeststhat the Mycobacterium tuberculosis and Mycobacterium bovis can be detected by PCR and the amplified158 bp DNA fragment can be utilized as a molecular probe.This probe can examine the Mycobacteriumtuberculosis and Mycobacterium bovis and distinguish them from other mycobacteria.

关 键 词：结核杆菌 聚合酶链反应 DNA探针 

分 类 号：R392－33[医药卫生—免疫学]