镰刀菌T-2毒素对培养软骨细胞的影响  

The Effect of T-2 Toxin on the Extracellular Matrix and the Enzymes of Mitochondrial Inner Membrane of Cultured Chicken Embryo Chondrocytes

作　　者：李生广[1] 孙珊[1] 吴莲英[1] 及惠芬[1] 洪杰 林治焕[1] 

机构地区：[1]中国科学院生物物理研究所,北京100101

出　　处：《生物化学与生物物理进展》1993年第5期364-368,共5页Progress In Biochemistry and Biophysics

基　　金："七.五"攻关项目75-62-03-08基金资助

摘　　要：在含有不同浓度 T-2 毒素培养液中离体培养鸡胚软骨细胞,当 T-2 浓度达到0.01ppm 时,可引起软骨细胞胞外基质胶原微原纤维减少,线粒体细胞色素 c 氧化酶和 H^+-ATP 酶的比活力下降.表明此浓度 T-2 毒素引起了软骨细胞结构与功能的明显改变.文章报道的方法可为检验大骨节病致病因子是否直接作用于大骨节病易侵蚀的软骨细胞提供了一种有效的实验手段.The chicken embryo chondrocytes were cultured in the media containing different concentrations ofT-2 toxin from Fusarium tricinctum,which was similar in structure with that from Fusarium speciesfound in grains from Kaschin-Beck disease area.When the concentration of the T-2 toxin rose to 0.01ppm,the collagen microfibrils in extracellular matrix of chicken embryo chondrocytes were significantlydecreased,and the activities of cytochrome c oxidase and H^+-ATPase of mitochondria were obviouslylower than that of the control.The results showed that 0.01 ppm the T-2 toxin induced the changes ofstructure and function of chicken embryo chondrocytes.The methods reported provided an effectivemeasure to examine if the factor which induced the Kaschin-Beck disease attacked on the target tis-sue——chondrocytes directly.

关 键 词：T-2毒素 大骨节病 镰刀菌 细胞 

分 类 号：R684.102[医药卫生—骨科学]