大鼠肾脏和其它组织中磷酸酪氨酸蛋白的研究  

Studies on P-tyr-Pr in Rat Kidney and Other Tissues

作　　者：张明志[1] 张光毅[1] 王迎伟[1] 赵升皓[1] 

机构地区：[1]徐州医学院生化教研室,徐州221002

出　　处：《生物化学杂志》1993年第2期213-218,共6页

摘　　要：本文采用P-tyr-BSA为免疫原免疫家无得抗血清。将纯化的IgG与HRP偶联,建立了P-tyr-Pr的ELISA法,并测定了正常大鼠肾脏等组织中P-tyr-Pr含量,其分布规律如下:上清中P-tyr-Pr含量高者,其颗粒部分则低,反之亦然;其中肾脏上清中含量远比其它组织(脾、肺、肝等)高。在此基础上,又研究了膜性肾炎大鼠肾脏P-tyr-Pr含量,发现其上清中的含量远远高于正常大鼠肾脏中的含量。Antiserum for P-tyr-Pr was obtained by immunizing rabbit with P-tyr-BSA. IgG was purified and coupled to HRP. ELISA for P-tyr-Pr was established and the contents of P-tyr-Pr in rat tissues were detected. The characteristic of P-tyr-Pr distribution in rat tissues was as follows: the tissue with high P-tyr-Pr content in the supernatant contained low P-tyr-Pr in the particle part; while the tissue with low P-tyr-Pr content in the supernatant contained high P-tyr-Pr in the particle part. The content of P-tyr-Pr in the kidney supernatant was much higher than in supernatant of any other rat tissue. We further studied the content of P-tyr-Pr in membranous nephritis rat kidney and found that the content of P-tyr-Pr in its supernatant was much higher than that in normal rat kidney supernatant.

关 键 词：磷酸酪氨酸 蛋白 肾脏 

分 类 号：Q593.9[生物学—生物化学]