溶葡球菌酶的纯化和某些性质  

Purification and Properties of Lysostaphin

作　　者：周润琦[1] 马力[2] 张培德[1] 阎栋材 杨瑞蓉 陈石根[1] 

机构地区：[1]复旦大学,上海200433 [2]宁夏医学院

出　　处：《生物化学杂志》1993年第3期358-363,共6页

摘　　要：1.应用本实验室构建的克隆菌株枯草杆菌0044进行了溶葡球菌酶的发酵生产,产量为150—200mg/L; 2.通过DEAE-纤维素,CM-纤维素和Sephadex G-50层析纯化了该酶;并以NaCl盐析方式,首次获得了该酶结晶; 3.测定了溶葡球菌酶的某些性质; 4.观察并讨论了溶葡球菌酶与溶菌酶等在溶菌作用上的相互加强。1. Recombinant strain B. subtilis 0044 was used for lysostaphin production with a yield of 150—200mg/L;2. Lysostaphin was purified by DEAE-cellulose, CM-cellulose, and Sephadex G-50 to apparent homogeneious as shown by SDS-PAGE; On the basis of purification, the lysostaphin was cryatalled by NaCl saltingout;3. Some properties of lysostaphin were determined;4. The potentiation of bacteriostasis on S.aureus and M. lysodeikticus of lysostaphin and lysozyme by each other was observed and the mechanism of the potentiation was also discussed.

关 键 词：溶葡萄球菌酶 提纯 

分 类 号：Q550.3[生物学—生物化学]