异搏定对小鼠肝癌腹水瘤细胞系PI-PLC活性的影响  

The Effect of Verapamil on Activities of PI-PLC from an Ascites Hepatoma Cell Clone

作　　者：姚晓丹[1] 王丽华[1] 赵宝昌[1] 曹玉霞[1] 顾淑珍[1] 

机构地区：[1]大连医学院生化教研室,大连116023

出　　处：《生物化学杂志》1993年第6期664-668,共5页

摘　　要：本文采用615近交系小鼠肝癌腹水瘤H_(ca)-F_(25)/CL-A_2细胞,测定钙拮抗剂异搏定作用前后磷脂酰肌醇特异的磷脂酶C(PI-PLC)活性的变化,并与同样处理的瘤株内钙恒稳的有关指标相比较。结果表明经异搏定处理后的A_2细胞PI-PLC活性显著降低,并与钙恒稳的指标基本呈现相应的平行变化趋势。提示PI-PLC可能参与肿瘤细胞内钙恒稳的变化过程,异搏定的作用可能与磷脂酰肌醇信号传导系统相关。H_(ca)-F_(25)/CL-A_2, a type of ascites hepatoma cell clones of 615 inbreeding strain mice was employed and the activities of phosphatidylinositol-specific phospholipase C (PI-PLC) were measured before and after treated with verapamil, and compared with some indexes of intracellular calcium homeostasis. The results showed that PI-PLC activities were decreased in the tumor cells after treated with verapamil. These changes occurred simultaneously with those changes indexes of calcium homeostasis. The results suggested that PI-PLC involved in changing the process of intracellular calcium homeostasis, and the effect of verapamil was closely associated with the phosphoinositide signaling pathway in the tumor cells.

关 键 词：磷脂酰肌醇 磷脂酶 异搏定 肝肿瘤 

分 类 号：R979.1[医药卫生—药品]