根瘤菌属几种同工酶的研究  

Studies of Several Isoenzymes about Rhizobium Sp.

作　　者：丁彦怀[1] 石勇民 马麟祥[3] 

机构地区：[1]辽宁大学生物系微生物室 [2]河北承德轻工业公司 [3]辽宁省农科院土肥所

出　　处：《生物技术》1993年第2期15-17,共3页Biotechnology

摘　　要：本文对根瘤菌属12株不同根瘤菌的过氧化物酶,谷氨酸草酰乙酸转氨酶及酯酶同工酶进行了测定,结果发现:所有菌株的过氧化物酶和谷氨酸草酰乙酸转氨酶均呈阴性。酯酶同工酶酶带数在快生型和慢生型根瘤菌之间存在明显的区别,在YMA培养基上,快生型根瘤菌仅有1—2条,迁移率在0.71—0.98之间变化;而慢生型根瘤菌却有4—6条酶带,迁移率在0.15—0.98之间变化,同一菌株在不同碳源上酯酶同工酶酶带数也发生显著变化。This paper is about the studies of peroxidase, glutamic-oxalacetic transaminase (GOT) and esterase in 12 strains of Rhizobium. The result were that all test strains had negative acivities about peroxi-dase and GOT. There was a quite difference about esterase between fast-and slow-growing rhizobium. In YMA medium, there were one or two bands of esterase and Ef was from 0.71 to 0.98 in fast-growing' rhizobium. There were, however, more bands of esterase from 4 to 6 and Ef had a wider change from 0.15 to 0.98 in slow-growing rhizobium. There were obvious changes of the number of esterase bands with different carbon resources to Rhizobium sp.

关 键 词：根瘤细菌属 同功酶 

分 类 号：S188[农业科学—农业基础科学]