长日照下农垦58_s叶中无特异mRNA丰富表达  

No Specific mRNA Abundantly Expressed in Nongken 58s Rice Leaves Under LD

作　　者：张力[1] 张启发[2] 

机构地区：[1]中国科学院上海生物化学研究所 [2]华中农业大学国家重点实验室

出　　处：《生物技术》1993年第6期12-16,共5页Biotechnology

基　　金：国家863-101主题基金;美国洛式基金

摘　　要：在本研究中,我们从光敏核不育水稻农垦58s育性对光周期敏感时期的叶片中制备出mRNA并合成了长日下的cDNA。进而构建了克隆效率达10~6克隆/μgcDNA的较完备的cDNA文库。然而,以长日和短日cDNA探针对该文库进行的+/-筛选未能筛选出长日下特异或相对丰富表达的mRNA的cDNA克隆。进而,以过量的(约50倍)短日mRNA与长日cDNA进行液相杂交,以富集长日特异的cDNA序列,富集后的长日cDNA经层析分离(Rot值为2.8×10~2)并标记为探针,与等量的长、短日cDNA杂交,但二者的杂交倍是无可辨差异,这些证据表明在农垦58s的育性光敏感时期长日下叶子无特异性的mPNA丰富表达。In the study, we synthesized LD (long daylength) cDNA molecules from mRNA templates of PSMS rice leaves at the stage of fertility photoperiod sensitivity. A cDNA library was successfully constructed with an efficiency of 10 6 colonies/ug cDNA. However, none cDNA clone corresponding to specific or relatively abundant mRNA under LD was screened out from the library by means of +/- screening method using LD and SD (short daylength) cDNAs as probes. Moreover, solution hybridization driven by excess (ca.50 fold) SD mRNA was carried out in an attempt to enrich specific cDNA fragments to LD. The enriched LD cDNA was isolated chromatographically (Rot value of 2.8 × 10 2) and labelled as a probe to hybride with equal amounts of LD and SD cDNAs.Yet no discernable differences of hybridzation signals between the dots were found.The evidences above argues the nonexist-ence of abundant mRNA specific to LD expressed in PSMS rice leaves at the stage of fertility photoperiod sensitivity.

关 键 词：cRNA 光敏核不育 水稻 日照 

分 类 号：S188[农业科学—农业基础科学]