淀粉麦芽寡糖酶的连续测活  

A CONTINUAL ASSAY OF MALTOTETRAOSE-FORMING AMYLASE FROM AN ALCALIGENES SPECIES

作　　者：赫荣乔[1] 赵康源[1] 

机构地区：[1]生物大分子国家重点实验室

出　　处：《生物物理学报》1993年第1期10-15,共6页Acta Biophysica Sinica

摘　　要：通过用糊精(含8-12个D-葡萄糖残基)做底物,以碘显色,在460nm监测光吸收的变化,测定淀粉麦芽四糖酶活力的结果表明:测活体系中含有一定量的钙离子.酶的测活曲线(在A_(460)<0.08的吸收范围)有较好的线性,该方法比间断法简便,快速,并且在测定淀粉麦芽四糖酶盐酸胍变性的失活动力学过程中.具有较好的重复性,可能成为淀粉麦芽寡糖酶测活的常用方法.A rapid and simple method for determination of Maltotetraose - forming Amylase, by using amylodextrin as substrate, was reported. The enzyme was incubated with dextrin and Ca2+ at 25℃, the absorbance at 460 nm was measured and the difference absorbance at 460 nm was estimated for determination of the enzyme specific activity. A linear relationship between absorbance and the consumed time was in a range of absorbance<0.08. The reproducibility is excellent for use in studying kinetics of the enzyme during GuHCl denaturation. It appears that this method could be used for determination of activities of amylases producing specifically oligosaccharide from starch.

关 键 词：测活 淀粉寡糖酶 麦芽寡糖酶 

分 类 号：Q617[生物学—生物物理学]