付里叶变换偏振红外光谱法研究紫膜LB膜内菌紫质的取向  

ORIENTATION OF BACTERIAHRODOPSIN IN PURPLE MEMBRANE LB FILM A POLARIZED INFRARED SPECTROSCOPIC STUDY

作　　者：梁圻 张极震[1] 杨景文[1] 胡坤生[1] 王敖金[1] 孙素琴[2] 胡鑫尧[2] 江龙[3] 

机构地区：[1]中国科学院生物物理研究所,北京100101 [2]清华大学化学系,北京100084 [3]中国科学院感光化学研究所,北京100101

出　　处：《生物物理学报》1993年第2期299-302,共4页Acta Biophysica Sinica

基　　金：国家自然科学基金

摘　　要：用付里叶变换偏振红外法测量了紫膜LB膜内菌紫质的酰胺Ⅰ、Ⅱ和A带的二色性,计算了菌紫质的α-螺旋取向.实验表明,紫膜内菌紫质的α-螺旋轴同膜法线的平均夹角为13°,即与膜平面的平均夹角为77°.The dichroism of the amide Ⅰ , Ⅱ , and A bands of purple membrane in LB film has been investigated by means of polarized infrared spectroscopy. The degree of orientation of α-helix of bacteriohrodopsin is estimated. The results indicate that in this film. the α-helices of the protein are titled at. on average. 13° with respect to the normal to the plate of the membrane, a reasonable value compared with that reported in literature.

关 键 词：紫膜 LB膜 红外偏振 

分 类 号：Q734[生物学—分子生物学]