活细胞免疫荧光法在免疫偶合物研究中的应用  

Application of Living Cellular Immunofluorescence Test in study of Immunoconjugate

作　　者：白丽[1] 王晶[1] 

机构地区：[1]大理医学院微生物学及免疫学教研室,大理671000

出　　处：《大理医学院学报》2001年第4期1-2,共2页Journal of Dali Medical College

基　　金：云南省教委科学研究基金资助课题(95/2136)

摘　　要：目的：探讨活细胞免疫荧光法在免疫偶合物制备过程中的应用价值。方法：将被测试物与活细胞作用，正常兔血清作封闭，加入ＦＩＴＣ—羊抗鼠ＩｇＧ，固定液固定后荧光显微镜观察。结果：含抗Ｔ淋巴细胞白血病单抗腹水、单抗纯化物及单抗偶合物的效价分别为１∶１０２４０、５ ｘ １０－８ｍｏｌ／Ｌ和 ５ ｘ １０－８ｍｏｌ／Ｌ。结论：活细胞免疫荧光法是一种简单、快速、结果易观察的方法，可用于单抗偶合物制备过程中的抗体活性测定。Objective: To explore the value of the living cell immunofluorescence test in the study of immunoconjugate. Methods: At first, the tseted substances were added in the living cells. Then the normal rabbit serum was mixed with them as a blocking factor. After that, FITC-goat -anti -mouse IgG was added. Finally, the cells were mixed by the fixing solution and observed using the fluorescence micro- scope. Results: The titers of the ascetics, the purified monoclonal antibody and the immunoconjugate were 1:10240, 5 × 10-8 mol/L and 5 ×10-8 mol/L, respectively. Conclusions: The method is simple, rapid and the result is easy to observe. It is suitable for detecting the activities of monoclonal antibodies in preparing immunoconjugates.

关 键 词：活细胞 免疫荧光 免疫偶合物 单克隆抗体 

分 类 号：R392[医药卫生—免疫学]