改良羊水染色体的制备技术  

A Moied Technique for the Preparation of Chromosome from Amniotic-fluid Cells

作　　者：曾爱群[1] 陈全娘[1] 冯英[1] 林雨[1] 李雄[1] 

机构地区：[1]广州医学院第一附属医院妇产科,广州510120

出　　处：《广州医学院学报》2001年第4期42-43,共2页Academic Journal of Guangzhou Medical College

摘　　要：目的:寻找一种简便、快速、效果稳定的羊水细胞染色体的制备方法。方法:总结１９年来我们遗传室羊水细胞培养的。作经验。结果和结论:通过增加培养液中Ｌ－谷氨酰胺的含量以及应用Ｈｅｐｅｓ,可使羊水细胞生长良好,而短时间高浓度的秋水侧素可获得较多的染色体分裂象。ve: To establish a simple,rapid and stable technique for the preparation of chromosome from amniotic -fluid cells. Methods: The experience in the cultivation of Amniotic fluid cells in the past 19 years in our genetics laboratory was summed up. Results and Conclusions: By increasing the amount of L-glutamine in culture medium and using Hepes,the amniotic fluid cells grew better. High concentration of colchicine in a short time period could achieve more chromosome mitosising phase.

关 键 词：羊水细胞 染色体 制备技术 改良 

分 类 号：R714.15[医药卫生—妇产科学]