NGF在成年猫脊髓和背根节分布的免疫组化研究  

Immunohistochemical Study on the Distribution of NGF in Adult Cats’ Spinal Cords and DRGs

作　　者：陈丽华[1] 陈钟[2] 戴莉萍[3] 

机构地区：[1]大理医学院组胚教研室,云南大理671000 [2]大理医学院成人教育学院,云南大理671000 [3]大理医学院非直属附属医院病理室,云南大理671000

出　　处：《大理医学院学报》2001年第3期1-2,共2页Journal of Dali Medical College

摘　　要：目的：探讨神经生长因子在成年猫脊髓背角和背根节的表达和意义。方法：采用抗神经生长因子多克隆抗体免疫组织化学方法，对 5只雄性家猫 L6节段脊髓和背根节作石蜡切片的 NGF免疫组织化学染色。结果：神经生长因子免疫阳性反应主要出现在脊髓背角Ⅰ、Ⅱ、Ⅲ板层外侧部的神经元胞核和胞浆中，以胞核为主；而在背根节阳性反应的神经元却寥寥无几。结论：神经生长因子可能参与脊髓神经元正常功能的维持。Objective: To probe into the expression and its significance of nerve growth factor(NGF) in adult cats’ spinal cords and their dorsal root ganglia (DRGs). Methods: Using immunohistochemical techniques labeled with anti－ NGF polyclonal antibodies to stain L6 segments of spinal cords and their DRGs in five adult cats. Results: The immunological positive reaction of NGF are mainly seen in the nucleus and cytoplasm of spinal cord lamina 1, 2 and 3, especially in the mucleus while scarcely seen in the neurons. Conclusion: NGF may be physiologically involved in the normal function maintenance of the spinal cord neurons.

关 键 词：神经生长因子 脊髓 背根节 免疫组化 

分 类 号：R338[医药卫生—人体生理学]