联合转染eNOS基因反义ET核酸对自体移植静脉内膜增生的影响  

The effects of ET antisence oligoneuleotide and eNOS genetic transfection on the intimal hyperplasia of venouse autografts

作　　者：王春喜[1] 段志泉[2] 黄志强[1] 梁发启[1] 宋清斌[2] 李荣[1] 

机构地区：[1]中国人民解放军总医院普通外科,北京100853 [2]中国医科大学第一临床学院血管外科,辽宁沈阳110001

出　　处：《中国现代普通外科进展》2001年第2期86-88,91,共4页Chinese Journal of Current Advances in General Surgery

摘　　要：目的 :探讨联合转染eNOS基因和反义ET核酸对自体移植静脉内膜增生的影响。方法 :制作2 0只自体颈静脉腹主动脉移植Wistar大鼠模型 ,实验组、对照组各 10只 ,实验组移植血管行腺病毒介导的eNOS溶液浸泡和反义ET核酸凝胶涂布 ,对照组仅行空载腺病毒溶液浸泡和凝胶涂布。术后 2周取出移植血管 ,利用病理学、免疫组织化学、RT PCR方法检测移植血管内膜厚度、管腔狭窄度、内膜VSMC数及PCNA阳性表达、血管ETmRNA、eNOSmRNA表达情况。结果 :实验组移植血管内膜厚度、管腔狭窄及VSMC数均较对照组减小或减少 ,PCNA阳性表达及ETmRNA表达较对照组减少 ,而eNOSmRNA表达则明显增加。结论 :联合转染NOS基因和反义ET核酸可有效地抑制移植静脉内膜的增生 ,是一种有效地防治移植静脉再狭窄的基因疗法。Objective: To investigate the effects of endothelin(ET) antisence oligoneuceotide and nitric oxide synthase (NOS)genetic transfection on the intimal hyperplasia of venous autografts.Methods: The external jugule veins were autografted into abdominal aorta arteries in 20 Wistar rats, which were divided evenly into experimental and control group at random. The transplanted veins of experimental group were immersed in the adenovirus-mediated eNOS gene solution for 15 minutes just before anastomosis and coated with ET antisence olioneucleotide glue gel just after anastomosis, while the control ones were only immersed the physiologic saline for 15 minutes but nothing to be coated. The transplanted vascular samples were taken at 2 weeks after operation. The intimal thinkness (IH), degree of restenosis(DR), expression of PCNA, ETmRNA, NOS mRNA were determined by histology, transcription polymerase chain reaction.Results: The IH, DR and the expressions of ETmRNA were decreased while the expressions of eNOS mRNA was increased contrary to control group.Conclusion: Transfection of ET antisence oligoneuleotide and NOS gene can inhibit the intimal hyperlisia of venouse autogrfts,it is a prospective and idea genetic prophylactic therapy to intimal hyperplasia.

关 键 词：内皮缩血管肽类 一氧化氮合酶 血管内膜 大鼠 基因治疗 

分 类 号：R543[医药卫生—心血管疾病] Q789[医药卫生—内科学]