用硅藻土提取肝脏中安定的方法研究  

The determination of diazepam in liver by solid—phase extraction with celite and UV derivative spectrophotometry

作　　者：玉红[1] 夏俊[1] 

机构地区：[1]中国刑警学院法医系,辽宁沈阳110035

出　　处：《刑事技术》2004年第6期8-9,共2页Forensic Science and Technology

基　　金：公安部资助科研项目 (NO .2 0 0 1932 310 1)

摘　　要：目的　建立以亲水性固相材料硅藻土为支持物分离提取肝中安定。方法　取 1 0 g肝匀浆用 6%高氯酸沉淀蛋白 ,离心 ,取上清液加入硅藻土柱中 ,用二氯甲烷洗脱。洗脱液 5 0℃水浴挥干后 ,用 4 0ml 1 0mol/L的盐酸溶解 ,测定其紫外二阶导数光谱。结果　提取率达 91 0 % ,变异系数 2 5 % ,检出限 0 2 5 μg/ g ,线性范围0 5— 5 0 μg/mL。 结论　该方法操作简单、实用 ,杂质含量低 。Objective To present a method of using celite to extract the diazepam in liver and using UV derivative spectrophotometry to analyze the diazepam. Method Liver homogenate(1g) was shaken with 6% perchLoric acid then centrifuged; the supernatant solution was added celite column and elution was effected with dichloromethane. The eluate was evaporated and the residue was redissolved in 4ml 1.0mol/L HCl solution. The second-derivative spectrum was measured by scanning between 220nm to 280nm. Result The detection limite of diazepam in liver was below 0.25μg/g.The recoverie was 91.0% and the relative standard deviation of recoverie was 2.5%. The linear range of determination was 0.5～5.0μg/ml. Conclusion The method can be used as a routine analytical medthod in forensic medicine.

关 键 词：安定 肝脏 方法研究 水浴 洗脱液 蛋白 上清液 法医毒物分析 支持 硅藻土 

分 类 号：D919[医药卫生—法医学]