Influence of Ureaplasma urealyticum infection on the sperm egg binding associated molecule,sulfogalactosylglycerolipid  

作　　者：Ting-TingWu Mei-GeLu Yan-QinHu Qiang-SuGuo ChenXu 

机构地区：[1]FacultyofHistology＆Embryology,ShanghaiSecondMedicalUniversity,Shanghai200025,China

出　　处：《Asian Journal of Andrology》2004年第4期354-354,共1页亚洲男性学杂志（英文版）

摘　　要：Aim: To study the influence of Ureaplasma urealyticum (Uu) infection on the sperm egg binding associated molecule, sulfoga-lactosylglycerolipid (SGG). Methods: Epididymal sperm was collected from adult mice. The sperm suspension was randomly divided into 4 groups: the Uu group (coincubated with Uu suspension), the medium group (coincubated with Uu medium), the normal group and the PRS group. Indirect immunofluorescence technique was used to localize SGG on the sperm membrane and to observe the influence of Uu on SGG. Results: In the epididymal sperm, SGG was localized at the head plasma membrane overlying the acroso-mal region. The SGG positive rate of the sperm coincubated with Uu medium was 82.0 %, while the SGG positive rate of those coincubated with Uu suspension was reduced to 39.0 % (P = 0.001). Conclusion: Uu can adhere to the sperm surface. SGG might be a membrane receptor on the sperm surface for Uu infection of the mammalian male genital tract. The blockage of SGG by Uu might be one of the molecular mechanisms correlated with male infertility induced by Uu infection.Aim: To study the influence of Ureaplasma urealyticum (Uu) infection on the sperm egg binding associated molecule, sulfoga-lactosylglycerolipid (SGG). Methods: Epididymal sperm was collected from adult mice. The sperm suspension was randomly divided into 4 groups: the Uu group (coincubated with Uu suspension), the medium group (coincubated with Uu medium), the normal group and the PRS group. Indirect immunofluorescence technique was used to localize SGG on the sperm membrane and to observe the influence of Uu on SGG. Results: In the epididymal sperm, SGG was localized at the head plasma membrane overlying the acroso-mal region. The SGG positive rate of the sperm coincubated with Uu medium was 82.0 %, while the SGG positive rate of those coincubated with Uu suspension was reduced to 39.0 % (P = 0.001). Conclusion: Uu can adhere to the sperm surface. SGG might be a membrane receptor on the sperm surface for Uu infection of the mammalian male genital tract. The blockage of SGG by Uu might be one of the molecular mechanisms correlated with male infertility induced by Uu infection.

关 键 词：male infertility Ureaplasma urealyticum sulfogalacto-sylglycerolipid sperm egg binding 

分 类 号：R698.2[医药卫生—泌尿科学]