放射性核素内照射诱发的DNA损伤效应  

DNA Damage Induced by Radionuclide Internal Irradiation.

作　　者：崔凤梅[1] 赵经涌[1] 洪承皎[1] 劳勤华[1] 王六一[1] 杨淑琴[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院,江苏苏州215007

出　　处：《中国辐射卫生》2004年第4期243-245,共3页Chinese Journal of Radiological Health

摘　　要：目的　研究晚期混合裂变产物内照射对大鼠外周血单个核细胞 (Peripheralbloodmononuclearcell,PBMC)DNA的损伤作用。方法　雄性Wistar大鼠经不同累积剂量和不同剂量率的晚期混合裂变产物内照射后 ,获得PBMC单细胞悬液 ,采用单细胞凝胶电泳 (Singlecellgelelectrophoresis ,SCGE)技术 ,检测细胞DNA链的断裂改变。结果　经晚期混合裂变产物作用后 ,各照射组PBMCDNA链断裂的迁移长度增加 (P <0 .0 5 ) ,显示良好的剂量效应关系和剂量率效应关系 ,均符合直线模型。结论　在本实验的内照射剂量下 ,晚期混合裂变产物能引起PBMC的DNA损伤 ,且呈现良好的剂量效应关系和剂量率效应关系。Objective To study the DNA damage of peripheral blood mononuclear cell (PBMC) in rats exposed to radionuclide internal irradiation. Methods The radionuclides were injected into the rats and single cell gel electrophoresis(SCGE) was performed to detect the length of DNA migration in the rat PBMC. Results DNA migration in the rat PBMC increased with accumulative dose or dose-rate. It showed good relationship of dose vs. response and of dose-rate vs. response,both relationship could be described as linear models. Conclusion Radionuclide internal irradiation could cause DNA damage in rat PBMC.

关 键 词：PBMC 内照射 晚期 DNA损伤 剂量效应关系 诱发 DNA链断裂 裂变产物 放射性核素 迁移 

分 类 号：R818[医药卫生—放射医学] R144.1[医药卫生—临床医学]