培养基组分及pH值对梨花粉萌发和花粉管生长的影响  

Effects of Medium Components and pH on Pollen Germination and Tube Growth in Pear (Pyrus pyrifolia)

作　　者：张绍铃[1] 陈迪新[1] 康琅[1] 汪玲[1] 

机构地区：[1]南京农业大学园艺学院,南京210095

出　　处：《西北植物学报》2005年第2期225-230,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目 (30270926 );高校博士学科点专项科研基金 (20010307012 )

摘　　要：采用花粉液体培养法研究不同培养基组分和pH值对梨花粉萌发和花粉管生长影响.结果表明:培养基内硼酸、氯化钙、PEG-4000、蔗糖、葡萄糖、麦芽糖、山梨醇、果糖在一定浓度范围内,对花粉萌发及花粉管生长起促进作用,但超过一定浓度时起抑制作用;最适的培养基组分为:30mmol/LMES、0.01%硼酸、0.03%CaCl2·2H2O、15%PEG-4000、5%果糖 或5%山梨醇、10%蔗糖 ,最适的pH值为6.5.在该培养基内梨花粉萌发率约为59.2%,花粉管生长长度为966.3μm.The effects of medium components and pH on pollen germination and tube growth in pear by cultivating pollens in liquid medium were studieds. The results showed that pollen germination and tube growth were promoted by the components and pH at certain values. Beyond values both pollen germination and tube growth were inhibited. 30　mmol/L MES, 0.01%　H_3BO_3, 0.03% CaCl_2·2H_2O, 15% PEG-4000, 5% D-fructose (or 5% sorbitol,10% sucrose), pH 6.5, were optimal for pollen germination and tube growth. In the conditions, pollen germination rate and tube length were 59.2% and 966.3 μm,respectively.

关 键 词：梨 培养基组分 花粉萌发 花粉管生长 PH值 

分 类 号：Q945.15[生物学—植物学]