川芎嗪对同基因骨髓移植小鼠骨髓中VCAM-1/VLA-4表达的影响  

Effects of Ligustrazine on Expression of VCAM-1/VLA-4 in Syngenic Bone Marrow Transplantation of Mice

作　　者：吴宁[1] 孙汉英[1] 刘文励[1] 何莉[1] 孟凡凯[1] 李春蕊[1] 刘丹[1] 徐惠珍[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院血液内科,武汉430030

出　　处：《中国实验血液学杂志》2005年第1期20-24,共5页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目;编号 3 9870 92 6

摘　　要：为了探讨同基因骨髓移植 (BMT)小鼠骨髓细胞表面黏附分子VCAM 1 VLA 4 (CD4 9d)的表达及川芎嗪对其影响 ,取健康BALB c小鼠 ,随机分为 3组 :正常组 (不做处理 ) ,骨髓移植对照组 (简称BMT组 )和骨髓移植 +川芎嗪治疗组 (简称川芎嗪组 )。BMT组和川芎嗪组分别胃饲生理盐水 0 .2ml 只和川芎嗪注射液每次 2mg 只 ,2次 天。在BMT后第 7、14、2 1、2 8天处死小鼠 ,采用免疫组织化学方法检测骨髓基质细胞VCAM 1蛋白表达水平 ,用RT PCR检测骨髓基质细胞VCAM 1mRNA表达水平 ;用流式细胞术检测骨髓有核细胞表面VLA 4的表达水平。结果发现 :BMT后第 7、14、2 1、2 8天川芎嗪组VCAM 1 VLA 4的表达水平、骨髓有核细胞计数均高于BMT组 (P <0 .0 5或P <0 .0 1)。结论 :川芎嗪能提高BMT小鼠骨髓造血细胞和基质细胞黏附分子的表达 ,促进造血干 祖细胞的归巢 ,加速移植后骨髓的造血重建。To explore the effect of ligustrazine on the expression of adherent molecule VCAM-1/VLA-4 of bone marrow cells in syngenic bone marrow transplantation (BMT) mice, the mice were divided into 3 groups:normal group (which received no treatment), BMT control group and ligustrazine-treated groups. BMT mouse models were established. The BMT control group and the ligustrazine-treated group were orally administered 0.2 ml saline per mouse and 2 mg ligustrazine per mouse, respectively, twice a day. On the day 7,14,21,28 after BMT, mice were respectively killed. Bone marrow nucleated cells were detected, and then the expression of VCAM-1/VLA-4 was assayed by immunohistochemistry, RT-PCR and flow cytometry analysis, respectively. The results showed that in ligustrazine-treated group, the accounts of bone marrow nucleated cells on the day 7,14,21,28 after BMT were all higher than that in BMT control group. The expression level in the ligustrazine-treated group was significantly higher than that in the BMT control group (P<0.05 or P<0.01). It is concluded that ligustrazine can enhance VCAM-1/VLA-4 expression in bone marrow after syngenic bone marrow transplantation in mice, which may be related to the mechanisms underlying the ligustrazine accelerating hematopoietic reconstitution in allogenic bone marrow transplantation.

关 键 词：川芎嗪 骨髓移植 造血重建 VCAM-1 VLA-4 

分 类 号：R457.7[医药卫生—治疗学] R979.1[医药卫生—临床医学]