间充质干细胞来源的成骨细胞具有免疫调控能力  

Osteoblasts Derived from Mesenchymal Stem Cells Harbor Immunoregulatory Effect

作　　者：江小霞[1] 张毅[1] 李秀森[1] 吴英[1] 于晓妉[1] 唐佩弦[1] 毛宁[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850

出　　处：《中国实验血液学杂志》2005年第1期50-53,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金(30271245;30470640);国家973项目(G1999054302);国家863项目(2003AA205170)

摘　　要：为了探讨来源于间充质干细胞 (mesenchymalstemcell,MSC)的成骨细胞的免疫调控能力 ,将MSC在成骨诱导体系中诱导分化 1周后用MTT法检测细胞生长变化 ,流式细胞术鉴定其免疫表型改变 ,淋巴母细胞转化实验观察其免疫调控能力。结果表明 ,MSC在向成骨诱导过程中细胞生长迅速 ,明显快于未诱导的MSC ,细胞表型未发生明显变化 ,即CD73,CD10 5 ,CD4 4 ,CD2 9等仍为强阳性 ,而造血细胞的表面标志CD34,CD4 5和参与免疫反应的细胞表面分子HLA DR ,CD86等仍为阴性。淋巴母细胞转化实验结果显示 ,源于MSC的成骨细胞具有免疫调控能力 ,且随着成骨细胞量的增多 ,抑制T细胞增殖能力越强。结论 :MSC来源的成骨细胞具有免疫调控能力。In an attempt to study the immunoregulatory effect of osteoblasts derived from mesenchymal stem cells (MSC), MSC was induced to differentiate into osteoblasts for one week. The growth pattern and the phenotype were evaluated by MTT and flow cytometry respectively. The immunoregulatory effect was tested by the inhibitory effect on T cell proliferation. The result showed that during the differentiation cells grew fast and there was no significant change in the phenotypes but keeping CD73, CD105, CD44, CD29 positive and CD34, CD45, HLA-DR, CD86 negative. Osteocyte derived from MSC also showed immunosuppressive effect on T cell proliferation in a dose-dependent manner. It is concluded that osteoblasts derived from MSC also harbord immunoregulatory effect.

关 键 词：间充质干细胞 成骨细胞 免疫调控 

分 类 号：R392.4[医药卫生—免疫学]