放烧复合伤大鼠血清对小鼠骨髓造血祖细胞的影响  

Effects of Blood Serum from Rats with Combined Radiation-Thermal Injury on the Bone Marrow Hematopoietic Progenitor Cells Growth

作　　者：冉新泽[1] 粟永萍[1] 郑怀恩[1] 郭朝华[1] 刘都户[1] 周燕虹[1] 刘晓宏[1] 艾国平[1] 

机构地区：[1]第三军医大学预防医学院全军复合伤研究所

出　　处：《中国实验血液学杂志》2005年第1期121-125,共5页Journal of Experimental Hematology

基　　金：国家自然科学基金重点项目资助;编号 3 0 2 3 0 3 60

摘　　要：以单纯烧伤、放射损伤为对照 ,观察放烧复合伤大鼠血清对骨髓造血祖细胞的影响及细胞因子的变化。用6 0 Coγ射线全身照射 12Gy ,5kW溴钨灯致大鼠背部 30 %全身体表面积Ⅲ度烧伤。伤后 3、12、2 4、4 8、72和 96小时无菌抽取大鼠血清。按 10 μg m1蛋白加入到骨髓红系或粒系培养体系中培养。伤后 2 4小时 ,应用ELISA法检测血清TNFα水平 ,应用放射免疫法检测IL 6含量。结果显示 ,无论烧伤或放烧复合伤血清组 ,伤后 3、12、2 4、4 8、72和 96小时的CFU E、BFU E和CFU GM培养集落数 ,均显著高于正常对照组 (P <0 .0 1) ,且以伤后 2 4小时最高 ,烧伤组高达 342 .8%、2 6 1.6 %和 2 2 8.4 % ;复合伤组为 2 5 2 .4 %、2 0 5 .1%和 174 .2 %。但当加入放射损伤大鼠血清时 ,CFU E、BFU -E和CFU GM集落数生长较少 ,最高为 12 .7%。同时 ,烧伤组和复合伤组血清TNFα和IL 6水平显著高于正常对照组 ,更高于放射损伤组。结论 :烧伤或放烧复合伤后的血清对骨髓红系和粒系造血均有明显的促进作用 ,放射损伤后的血清则有明显的抑制作用 ;该作用与血清细胞因子变化有关。To observe the effects of blood serum from rats with radiation injury,thermal injury and combined radiation-thermal lesions on growth of hematopoietic progenitor cells and the change of their serum cytokine levels, total body irradiation of rats was perfomed with 12 Gy γ ray from a 60Co source, and 30% total body surface area III degree thermal lesion on the back was inflicted with a 5 kW bromotungsten lamp. The blood serum from these animals was collected at 3, 12, 24, 48, 72 and 96 hours after injury. Then the blood serum was added to the culture medium of erythrocyte progenitor cells(CFU-E, BFU-E) or granulocyte-macrophage progenitor cells (CFU-GM) at final concentration of 10 μg/ml. The results showed that the colony number of CFU-E,BFU-E and CFU-GM formed after addition of the blood serum from rats with thermal or combined radiation-thermal jnjury was significantly higher than that from normal rats at 3, 12, 24, 48, 72 and 96 hours after injury and reached its peak value at 24 hours after injury (342.8,261.6 and 228.4% respectively from burned rats, 252.4, 205.1 and 174.2% respectively from rats with combined radiation-thermal injury as compared with that of normal rats). However, a few CFU-E, BFU-E or CFU-GM formation was found after addition of the blood serum from irradiated rats. At the same time, the level of TNFα and IL-6 in serum of burn group and combined radiation-thermal injury group was markedly higher than that of normal group, even more higher than that of irradiation injury group (P<0.01). It is concluded that the blood serum from rats with thermal lesion or combined radiation-thermal injury improves the growth of erythrocyte and granulocyte progenitor cells. On the contrary, the blood serum from the irradiated rats shows the inhibiting effects, definitely related to their serum cytokines changes.

关 键 词：放射损伤 烧伤 放烧复合伤 血清 造血祖细胞 细胞因子 

分 类 号：R818[医药卫生—放射医学] R644[医药卫生—临床医学]