高效液相色谱法测定5种不同产地丹参中丹参素和原儿茶醛含量  

Content Determination of Danshensu and Protocatechuic Aldehyde in Five Species of Salvia Miltiorrhiza by HPLC

作　　者：黄敬群[1] 张秀巧[1] 郭军[1] 张莉[2] 

机构地区：[1]中国人民解放军第252医院,河北保定071000 [2]中国人民解放军第四军医大学西京医院

出　　处：《安徽中医学院学报》2005年第1期37-40,共4页Journal of Anhui Traditional Chinese Medical College

摘　　要：目的 :测定 5种产地丹参中丹参素、原儿茶醛的含量。方法 :采用高效液相色谱法 ,Kro masil分析柱 (2 5 0mm× 4 6mm ,5 μm ;流动相 :甲醇 10g/L冰醋酸 2 0 80 ;紫外检测波长 :2 80nm ;流速 :1.0ml/min。结果 :丹参素在 4 .2～ 33.6 μg/ml,原儿茶醛在 0 .36～ 1.80 μg/ml范围内线性关系良好 ,丹参素和原儿茶醛最低检测限分别为 10 .5ng和 0 .6ng ,回收率分别为 99.36 % (RSD为 1.2 6 % )和 97.5 4% (RSD为 0 .81% )。结论 :该方法简便、灵敏、准确 ,样品处理简便易行 ,在考察评价丹参药材质量上有实用价值。不同产地的丹参 ,其丹参素和原儿茶醛含量有一定差异。Objective:To determine the contents of danshensu and protocatechuic aldehyde in five species of Salvia miltiorrhiza. Method: The HPLC was employed. Chromatographic conditions included Kromasil column (250 mm×46 mm,5μm), the mobile phase consisted of methanol and 10 g/L acetic acid (2080), the UV detector was set at 280 nm and the flow rate was 1.0 ml/min. Result: The standard curve of Danshensu was linear within the range of 4.2～33.6 g/ml, while protocatechuic aldehyde was linear within the range of 0.36～1.8 g/ml . The lowest detectable limit of Danshensu and protocatechuic aldehyde was 10.5 ng and 0.6 ng, respectively; the recovery rate was 99.36% (RSD=1.26%) and 97.54% (RSD=0.81%) , respectively. Conclusion: This method is convenient, sensitive and accurate, it is satisfactory for the quality control of Salvia miltiorrhiza; the contents of danshensu and protocatechuic aldehyde in five species of Salvia miltiorrhiza are different.

关 键 词：丹参 丹参素 原儿茶醛 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]