一种新的亲合素法分离孕妇外周血中的有核红细胞  

A lectin-based method for isolation of nucleated red blood cell from maternal blood

作　　者：唐爱兰[1] 刘国仰[1] 夏晓艳[2] 高春生[1] 刘歌[1] 张俊荣[3] 张学[1] 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所医学遗传学系,北京100005 [2]北京市妇产医院,北京100006 [3]北京医院,北京100730

出　　处：《中国优生与遗传杂志》2005年第1期13-15,共3页Chinese Journal of Birth Health & Heredity

基　　金：美国中华医学基金会资助课题

摘　　要：目的　为了分离孕妇外周血中的有核红细胞用于非损伤性产前诊断。方法　采用了一种新的亲合素法 ,用红细胞表面半乳糖基特异的凝集素 (大豆凝集素 )来选择富集孕妇外周血中的有核红细胞。结果　从 11份 2 .5ml孕 14 + ～ 15 +w的孕妇外周血中都分离到了有核红细胞 ,平均每份血样得到有核红细胞 2 .8个。结论　本方法实验成本低 ,操作简单 ,对于非损伤性产前诊断的研究和推广都具有重要的参考价值。Objective: To isolate nucleated red blood cells (NRBCs) from maternal blood. Methods: A new technique using galactose-specific lectin was adopted, which based on galactose expression on the surface of NRBC. Results: NRBCs were successfully isolated from 11 blood samples of pregnant women at 14 +～15 + gestational weeks. The average of 2.8 NRBCs were obtained from 2.5ml blood sample. Conclusion: A simple and sensitive method was presented for isolation of NRBCs in maternal blood, which may be helpful for clinical application of noninvasive prenatal diagnosis.

关 键 词：大豆凝集素 双密度梯度离心 有核红细胞 非损伤性产前诊断 

分 类 号：R714.55[医药卫生—妇产科学]