血管紧张素系统基因多态性与支架内再狭窄的相关性研究  

Association of renin-angiotensin system gene polymorphisms and the incidence of in-stent restenosis

作　　者：郭锋[1] 陈丽君[1] 谢佐俊[1] 刘雯[1] 李锦燕[1] 左伋[1] 

机构地区：[1]复旦大学上海医学院细胞与遗传医学系,200032

出　　处：《中国优生与遗传杂志》2005年第1期18-20,23,共4页Chinese Journal of Birth Health & Heredity

摘　　要：目的　探讨肾素 -血管紧张素系统 (RAS)中血管紧张素原 (AGT)、血管紧张素Ⅰ转换酶 (ACE)和受体 (AT1R)的基因多态性与PTCA加支架置入术后再狭窄 (ISR)发生的相关性。方法　 10 3例行PTCA加支架置入术的患者 ,分为狭窄组和未狭窄组 ,应用PCR -RFLP及AFLP方法对ACE和AT1R基因进行基因分型并分别计算基因型频率 ,卡方检验确定两组间差异的显著性。结果　ACE基因DD型患者比DI型、II型患者有较高的再狭窄发生率 (χ2 =9.75 9,P =0 .0 0 8) ;AT1R(A116 6C)基因多态与ISR无明显相关性 (χ2 =0 .75 9,P =0 .372 )。结论　RAS系统中ACE(DD)基因多态型可能是上海汉族冠心病人群PTCA后ISR发生的遗传指标 ,而D等位基因则可能是ISR的预测因子。Objective:To study the relationship between renin-angiotensin system(RAS) related gene polymorphisms, including angiotensinogen(AGT)M235T, angiotensin converting enzyme(ACE) insertion/deletion (I/D), angiotensin Ⅱ type 1 receptor(A1166C), and in-stent restenosis (ISR) after Percutaneous Transluminal Coronary Angioplasty (PTCA). Methods: 103 patients with CHD, underwent coronary stenting, were randomly selected and divided into ISR group and non-ISR group, PCR-RFLP and AFLP methods were used to type AGT(M235T) genotype, the insertion/deletion polymorphism at intron 16 of ACE gene, AT1R A1166C gene polymorphism and the genotype frequency of each of them was calculated, the difference between the two groups was examed by Chi-squared test. Results:The patients with ACE (D/D) genotype have higher frequency of restenosis than those with ID and Ⅱ genotype(χ 2 =9.759,P=0.008). There was no difference of the genotype frequency of AT1R(A1166C) gene polymorphism between the two groups. Conclusion:In the group of CHD patients, the ACE (D/D) polymorphism promoted the progress of in-stent restenosis and was of clinical significance. D allele may be a genetic predictor of ISR after PTCA. Among the other potential variables examined did not correlate with in-stent restenosis.

关 键 词：肾素-血管紧张素系统 支架内再狭窄 基因多态性 

分 类 号：R541.4[医药卫生—心血管疾病]