水稻光温敏雄性不育系培矮64S的抽穗期基因型分析  

Analysis of Heading Time Genotype for a Rice Photoperiod and Thermo - sensitive Male Sterile Line PeiAi64S

作　　者：徐俊锋[1] 江玲[1] 王国刚[1] 刘世家[1] 陈亮明[1] 王春明[1] 罗林广[1] 万建民[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,江苏省植物基因工程技术研究中心,南京210095

出　　处：《Acta Genetica Sinica》2005年第1期57-65,共9页

基　　金：国家 8 63计划 (编号 :2 0 0 1AA2 410 2 4);江苏省科技厅攻关项目 (编号 :BE2 0 0 13 0 5 )~~

摘　　要：利用抽穗期感光性近等基因系EG0～EG7、ER～LR及T6 5～T6 5m对培矮 6 4S抽穗期基因型进行分析表明 ,培矮 6 4S在E1、E2 、E3 位点分别带有E1、e2 、E3 基因 ,在Se 1位点带无感光功能的Se 1e 基因 ,在Ef位点带有显性早熟基因Ef 1。进一步用抽穗期QTL近等基因系NIL(Hd1)和NIL(Hd4 )进行的研究证实 ,培矮 6 4S带有显性感光基因E1和无感光功能的Se 1e 基因。同时 ,用QTL近等基因系日本晴和NIL(Hd2 )～NIL(Hd6 )研究表明培矮 6 4S带有能抑制E1基因表达的隐性感光抑制基因i Se 1。文中对培矮 6PeiAi64S,a photoperiod and thermo - sensitive genic male sterile,has been wildly applied to hybrid rice seed production in China,but its photoperiod-sensitivity gene for heading date in this sterile line was still unknown.This definitely limited the further use of this sterile line in breeding practice and re-production of hybrid seeds.To solve this problem,using heading time nearly isogenic lines EG0～EG7、ER～LR、T65～T65m and six heading date QTL-isogenic lines,NIL (Hd1) ～NIL (Hd6) with the genes of Nipponbare but Hd1 ～ Hd6 genes from Kasalath respectively,we performed a genetic analysis of PeiAi64S with special reference to photoperiod-sensitivity loci,in natural long days at Nanjing(32° N) where the average day-length is about 14 h and in natural short days at Linshui county,Hainan province(18° 29′N),where the average day-length is about 11.6 h during the course of rice growing respectively.The F_1 and F_2 generations from the crosses ‘PeiAi64S×heading time nearly isogenic lines’ were subjected to genetic analyses.Experimental results showed that PeiAi64S carries photoperiod-sensitivity allele gene E_1 and E_3 and dominant earliness gene Ef-1 in E_1 and E_3 and Ef-1 loci,respectively,and a photoperiod insensitivity allele Se-1~e in Se-1 locus.Meanwhile,the photoperiod-sensitivity gene E_1 and photoperiod-insensitivity gene Se-1~e in PeiAi64S were also identified by crossing with the NIL(Hd1)and NIL(Hd4).In addition,a recessive inhibitor for photoperiod-sensitive gene E_1 or Se-1~n and other modified photoperiod-sensitive genes: i-Se-1,E_3,Hd3(En-Se-1),Hd5 and Hd6,were identified in PeiAi64S by crossing with QTL nearly isogenic lines: NIL(Hd2),NIL(Hd3),NIL(Hd5)and NIL(Hd6),The results indicated that the genotype of PeiAi64S’s heading date was: E_1E_1e_2e_2E_3E_3Se-1~eSe-1~eEf-1Ef-1 i-Se-1 i-Se-1.

关 键 词：水稻(Oryza safiva L.) 近等基因系 抽穗期 感光基因 显性早熟基因 

分 类 号：S511[农业科学—作物学]